19667393
not annotated - annotated - LINNAEUS only
Methanoregula formicica sp. nov., a methane-producing archaeon isolated from methanogenic sludge.
A novel methane-producing archaeon, strain SMSP(T), was isolated from an anaerobic, propionate-degrading enrichment culture that was originally obtained from granular sludge in a mesophilic upflow anaerobic sludge blanket (UASB) reactor used to treat a beer brewery effluent. Cells were non-motile, blunt-ended, straight rods, 1.0-2.6 mum long by 0.5 mum wide; cells were sometimes up to 7 mum long. Asymmetrical cell division was observed in rod-shaped cells. Coccoid cells (0.5-1.0 mum in diameter) were also observed in mid- to late-exponential phase cultures. Growth was observed between 10 and 40 ^0C (optimum, 30-33 ^0C) and pH 7.0 and 7.6 (optimum, pH 7.4). The G+C content of the genomic DNA was 56.2 mol%. The strain utilized formate and hydrogen for growth and methane production. Based on comparative sequence analyses of the 16S rRNA and mcrA (encoding the alpha subunit of methyl-coenzyme M reductase, a key enzyme in the methane-producing pathway) genes, strain SMSP(T) was affiliated with group E1/E2 within the order Methanomicrobiales. The closest relative based on both 16S rRNA and mcrA gene sequences was Methanoregula boonei 6A8(T) (96.3 % 16S rRNA gene sequence similarity, 85.4 % deduced McrA amino acid sequence similarity). The percentage of 16S rRNA gene sequence similarity indicates that strain SMSP(T) and Methanoregula boonei 6A8(T) represent different species within the same genus. This is supported by our findings of shared phenotypic properties, including cell morphology and growth temperature range, and phenotypic differences in substrate usage and pH range. Based on these genetic and phenotypic properties, we propose that strain SMSP(T) represents a novel species of the genus Methanoregula, for which we propose the name Methanoregula formicica sp. nov., with the type strain SMSP(T) (=NBRC 105244(T) =DSM 22288(T)).
Ann file
T1 Species 0 23 Methanoregula formicica
N1 Reference T1 Taxonomy:882104
T2 Species 143 147 SMSP
N2 Reference T2 Taxonomy:882104
T3 Species 1076 1080 SMSP
N3 Reference T3 Taxonomy:882104
T4 Species 1225 1249 Methanoregula boonei 6A8
N4 Reference T4 Taxonomy:358766
T5 Species 1423 1427 SMSP
N5 Reference T5 Taxonomy:882104
T6 Species 1435 1459 Methanoregula boonei 6A8
N6 Reference T6 Taxonomy:358766
T7 Species 1770 1774 SMSP
N7 Reference T7 Taxonomy:882104
T8 Species 1863 1886 Methanoregula formicica
N8 Reference T8 Taxonomy:882104
T9 Species 1918 1922 SMSP
N9 Reference T9 Taxonomy:882104