20044015
not annotated - annotated - LINNAEUS only
Characterizing the role of the microtubule binding protein Bim1 in Cryptococcus neoformans.
During sexual development the human fungal pathogen Cryptococcus neoformans undergoes a developmental transition from yeast-form growth to filamentous growth. This transition requires cellular restructuring to form a filamentous dikaryon. Dikaryotic growth also requires tightly controlled nuclear migration to ensure faithful replication and dissemination of genetic material to spore progeny. Although the gross morphological changes that take place during dikaryotic growth are largely known, the molecular underpinnings that control this process are uncharacterized. Here we identify and characterize a C. neoformans homolog of the Saccharomyces cerevisiae BIM1 gene, and establish the importance of BIM1 for proper filamentous growth of C. neoformans. Deletion of BIM1 leads to truncated sexual development filaments, a severe defect in diploid formation, and a block in monokaryotic fruiting. Our findings lead to a model consistent with a critical role for BIM1 in both filament integrity and nuclear congression that is mediated through the microtubule cytoskeleton.
Ann file
T1 Species 67 90 Cryptococcus neoformans
N1 Reference T1 Taxonomy:5207
T2 Species 124 129 human
N2 Reference T2 Taxonomy:9606
T3 Species 146 169 Cryptococcus neoformans
N3 Reference T3 Taxonomy:5207
T4 Species 701 714 C. neoformans
N4 Reference T4 Taxonomy:5207
T5 Species 730 754 Saccharomyces cerevisiae
N5 Reference T5 Taxonomy:4932
T6 Species 836 849 C. neoformans
N6 Reference T6 Taxonomy:5207
T7 Out-of-scope 212 217 yeast
N7 Reference T7 Taxonomy:4932