20154330
not annotated - annotated - LINNAEUS only
Gordonia humi sp. nov., isolated from soil.
A Gram-stain-positive, non-endospore-forming actinobacterium (CC-12301(T)) was isolated from soil attached to a spawn used in the laboratory to grow the edible mushroom Agaricus brasiliensis. Based on 16S rRNA gene sequence similarities, strain CC-12301(T) was shown to belong to the genus Gordonia and was most closely related to the type strains of Gordonia hydrophobica (97.6 % similarity), Gordonia terrae (97.5 %), Gordonia amarae (97.5 %) and Gordonia malaquae (97.4 %). The quinone system was determined to consist predominantly of menaquinone MK-9(H(2)), minor amounts of MK-8(H(2)) and MK-7(H(2)). The polar lipid profile consisted of the major compounds diphosphatidylglycerol and phosphatidylethanolamine, moderate amounts of two phosphatidylinositol mannosides and phosphatidylinositol and minor amounts of phosphatidylglycerol, three unidentified glycolipids, two phosphoglycolipids and a phospholipid. Mycolic acids were present. These chemotaxonomic traits and the major fatty acids, which were C(16 : 1) cis9, C(16 : 0), C(18 : 1) and tuberculostearic acid (10-methyl C(18 : 0)), supported the affiliation of strain CC-12301(T) to the genus Gordonia. The results of physiological and biochemical tests allowed clear phenotypic differentiation of strain CC-12301(T) from the most closely related Gordonia species. Strain CC-12301(T) therefore represents a novel species, for which the name Gordonia humi sp. nov. is proposed, with the type strain CC-12301(T) (=DSM 45298(T) =CCM 7727(T)).
Ann file
T1 Species 0 13 Gordonia humi
N1 Reference T1 Taxonomy:686429
T2 Species 107 115 CC-12301
N2 Reference T2 Taxonomy:686429
T3 Species 214 235 Agaricus brasiliensis
N3 Reference T3 Taxonomy:307931
T4 Species 290 298 CC-12301
N4 Reference T4 Taxonomy:686429
T5 Species 396 417 Gordonia hydrophobica
N5 Reference T5 Taxonomy:40516
T6 Species 439 454 Gordonia terrae
N6 Reference T6 Taxonomy:2055
T7 Species 465 480 Gordonia amarae
N7 Reference T7 Taxonomy:36821
T8 Species 494 511 Gordonia malaquae
N8 Reference T8 Taxonomy:410332
T9 Species 1177 1185 CC-12301
N9 Reference T9 Taxonomy:686429
T10 Species 1314 1322 CC-12301
N10 Reference T10 Taxonomy:686429
T11 Species 1381 1389 CC-12301
N11 Reference T11 Taxonomy:686429
T12 Species 1450 1463 Gordonia humi
N12 Reference T12 Taxonomy:686429
T13 Species 1507 1515 CC-12301
N13 Reference T13 Taxonomy:686429