20154332
not annotated - annotated - LINNAEUS only
Marinobacterium coralli sp. nov., isolated from mucus of coral (Mussismilia hispida).
A Gram-negative, aerobic bacterium, designated R-40509(T), was isolated from mucus of the reef builder coral (Mussismilia hispida) located in the Sao Sebastiao Channel, Sao Paulo, Brazil. The strain was oxidase-positive and catalase-negative, and required Na(+) for growth. Its phylogenetic position was in the genus Marinobacterium and the closest related species were Marinobacterium sediminicola, Marinobacterium maritimum and Marinobacterium stanieri; the isolate exhibited 16S rRNA gene sequence similarities of 97.5-98.0 % with the type strains of these species. 16S rRNA gene sequence similarities with other type strains of the genus Marinobacterium were below 96 %. DNA-DNA hybridizations between strain R-40509(T) and the type strains of the phylogenetically closest species of the genus Marinobacterium revealed less than 70 % DNA-DNA relatedness, supporting the novel species status of the strain. Phenotypic characterization revealed that the strain was able to grow at 15-42 ^0C and in medium containing up to 9 % NaCl. The isolate could be differentiated from phenotypically related species by several features, including its ability to utilize d-alanine, l-alanine, bromosuccinic acid, Beta-hydroxybutyric acid and alpha-ketovaleric acid, but not acetate or l-arabinose. It produced acetoin (Voges-Proskauer), but did not have esterase lipase (C8) or catalase activities. It possessed C(18 : 1)omega7c (35 %), summed feature 3 (iso-C(15 : 0) 2-OH and/or C(16 : 1)omega7c; 25 %) and C(16 : 0) (22 %) as major cellular fatty acids. The DNA G+C content was 58.5 mol%. The name Marinobacterium coralli sp. nov. is proposed to accommodate this novel isolate; the type strain is R-40509(T) (=LMG 25435(T) =CAIM 1449(T)).
Ann file
T1 Species 0 23 Marinobacterium coralli
N1 Reference T1 Taxonomy:693965
T2 Species 64 83 Mussismilia hispida
N2 Reference T2 Taxonomy:242724
T3 Species 135 142 R-40509
N3 Reference T3 Taxonomy:693965
T4 Species 198 217 Mussismilia hispida
N4 Reference T4 Taxonomy:242724
T5 Species 458 486 Marinobacterium sediminicola
N5 Reference T5 Taxonomy:518898
T6 Species 488 513 Marinobacterium maritimum
N6 Reference T6 Taxonomy:500162
T7 Species 518 542 Marinobacterium stanieri
N7 Reference T7 Taxonomy:49186
T8 Species 801 808 R-40509
N8 Reference T8 Taxonomy:693965
T9 Species 1678 1701 Marinobacterium coralli
N9 Reference T9 Taxonomy:693965
T10 Species 1777 1784 R-40509
N10 Reference T10 Taxonomy:693965
T11 Species 1790 1799 LMG 25435
N11 Reference T11 Taxonomy:693965
T12 Species 1804 1813 CAIM 1449
N12 Reference T12 Taxonomy:693965