20173002

not annotated - annotated - LINNAEUS only

Litoreibacter albidus gen. nov., sp. nov. and Litoreibacter janthinus sp. nov., members of the class Alphaproteobacteria isolated from the seashore.

Two Gram-negative, strictly aerobic, non-motile bacteria designated strains KMM 3851(T) and KMM 3842(T) were respectively isolated from a marine snail specimen (Umbonium costatum) and from surrounding sediments collected simultaneously from the shore of the Sea of Japan. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains KMM 3851(T) and KMM 3842(T) were affiliated with the Roseobacter lineage of the class Alphaproteobacteria as a separate phylogenetic line adjacent to the members of the genus Thalassobacter. These novel isolates shared 98.5 % 16S rRNA gene sequence similarity and 15 % DNA-DNA relatedness. The major isoprenoid quinone of both strains was Q-10 and polar lipids consisted of phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and aminophospholipids. In addition, strain KMM 3851(T) contained two unknown lipids, whereas strain KMM 3842(T) contained diphosphatidylglycerol. Fatty acid analysis revealed C(18 : 1)omega7c and C(16 : 0) as major components and small amounts of C(18 : 2). The DNA G+C contents were 60.4 mol% (KMM 3851(T)) and 58.5 mol% (KMM 3842(T)). Based on distinctive phenotypic characteristics, DNA-DNA hybridization data and phylogenetic distance, strains KMM 3851(T) and KMM 3842(T) should be classified as representatives of two novel species in a new genus, Litoreibacter gen. nov., with the type species Litoreibacter albidus sp. nov. (type strain KMM 3851(T) =NRIC 0773(T) =JCM 16493(T)) and a second species Litoreibacter janthinus sp. nov. (type strain KMM 3842(T) =NRIC 0772(T) =JCM 16492(T)).



Ann file

T1	Species 0 21	Litoreibacter albidus

N1 Reference T1 Taxonomy:670155

T2 Species 46 69 Litoreibacter janthinus

N2 Reference T2 Taxonomy:670154

T3 Species 227 235 KMM 3851

N3 Reference T3 Taxonomy:670155

T4 Species 243 251 KMM 3842

N4 Reference T4 Taxonomy:670154

T5 Species 312 329 Umbonium costatum

N5 Reference T5 Taxonomy:335737

T6 Species 498 506 KMM 3851

N6 Reference T6 Taxonomy:670155

T7 Species 514 522 KMM 3842

N7 Reference T7 Taxonomy:670154

T8 Species 984 992 KMM 3851

N8 Reference T8 Taxonomy:670155

T9 Species 1041 1049 KMM 3842

N9 Reference T9 Taxonomy:670154

T10 Species 1236 1244 KMM 3851

N10 Reference T10 Taxonomy:670155

T11 Species 1264 1272 KMM 3842

N11 Reference T11 Taxonomy:670154

T12 Species 1389 1397 KMM 3851

N12 Reference T12 Taxonomy:670155

T13 Species 1405 1413 KMM 3842

N13 Reference T13 Taxonomy:670154

T14 Species 1541 1562 Litoreibacter albidus

N14 Reference T14 Taxonomy:670155

T15 Species 1585 1593 KMM 3851

N15 Reference T15 Taxonomy:670155

T16 Species 1647 1670 Litoreibacter janthinus

N16 Reference T16 Taxonomy:670154

T17 Species 1693 1701 KMM 3842

N17 Reference T17 Taxonomy:670154