20173005

not annotated - annotated - LINNAEUS only

Thiomonas islandica sp. nov., a moderately thermophilic, hydrogen- and sulfur-oxidizing betaproteobacterium isolated from a hot spring.

A novel, hydrogen- and sulfur-oxidizing bacterium, designated strain 6C(T), was isolated from a hot spring in Graendalur, south-western Iceland. Cells of this organism were Gram-reaction-negative, rod-shaped and motile. The strain grew aerobically and was capable of chemolithotrophic growth on thiosulfate and hydrogen, heterotrophic growth on pyruvate, oxalate, acetate and on glutamate in the presence of yeast extract and mixotrophic growth on several organic compounds, thiosulfate and/or hydrogen. During growth on thiosulfate, the final product was sulfate, resulting in a drop in pH from 6.8 to 2.7. Heterotrophic growth on pyruvate was observed at pH 4-7 (optimum pH 4) and 35-50 ^0C (optimum 45 ^0C). The DNA G+C content was 65.2 mol%. As determined by 16S rRNA gene sequence analysis, strain 6C(T) represents a distinct species belonging to the class Betaproteobacteria and is most closely related to Thiomonas intermedia DSM 18155(T) and Thiomonas perometabolis DSM 18570(T). DNA-DNA hybridization between strain 6C(T) and Thiomonas intermedia DSM 18155(T) and Thiomonas perometabolis DSM 18570(T) gave relatedness values below 32 %. These results, together with physiological characteristics, showed that strain 6C(T) represents a novel species within the genus Thiomonas, for which the name Thiomonas islandica sp. nov. is proposed. The type strain is 6C(T) (=DSM 21436(T) =JCM 16107(T)).



Ann file

T1	Species 0 19	Thiomonas islandica

N1 Reference T1 Taxonomy:545537

T2 Species 88 107 betaproteobacterium

N2 Reference T2 Taxonomy:86027

T3 Species 207 209 6C

N3 Reference T3 Taxonomy:545537

T4 Species 941 943 6C

N4 Reference T4 Taxonomy:545537

T5 Species 1000 1018 Betaproteobacteria

N5 Reference T5 Taxonomy:86027

T7 Species 1050 1080 Thiomonas intermedia DSM 18155

N7 Reference T7 Taxonomy:926

T9 Species 1088 1121 Thiomonas perometabolis DSM 18570

N9 Reference T9 Taxonomy:928

T10 Species 1163 1165 6C

N10 Reference T10 Taxonomy:545537

T12 Species 1173 1203 Thiomonas intermedia DSM 18155

N12 Reference T12 Taxonomy:926

T14 Species 1211 1244 Thiomonas perometabolis DSM 18570

N14 Reference T14 Taxonomy:928

T15 Species 1363 1365 6C

N15 Reference T15 Taxonomy:545537

T16 Species 1443 1462 Thiomonas islandica

N16 Reference T16 Taxonomy:545537

T17 Species 1504 1506 6C

N17 Reference T17 Taxonomy:545537

T18 Species 1512 1521 DSM 21436

N18 Reference T18 Taxonomy:545537

T19 Species 1526 1535 JCM 16107

N19 Reference T19 Taxonomy:545537

T20 Out-of-scope 546 551 yeast

N20 Reference T20 Taxonomy:147537 Saccharomycotina

#1 AnnotatorNotes T5 TODO check specificity

#6 AnnotatorNotes T2 TODO check specificity