20173008
not annotated - annotated - LINNAEUS only
Anoxybacillus tengchongensis sp. nov. and Anoxybacillus eryuanensis sp. nov., facultatively anaerobic, alkalitolerant bacteria from hot springs.
Two novel thermophilic, spore-forming bacterial strains, T-11(T) and E-112(T), were isolated from hot springs in Tengchong and Eryuan counties of Yunnan province in south-west China. The strains were Gram-stain-positive rods, occurring singly or in chains. Growth of strain T-11(T) was observed between 30 and 75 ^0C (optimum 50 ^0C) and at pH 7-11 (optimum pH 8.5), while the temperature range for strain E-112(T) was 35-70 ^0C (optimum 55 ^0C) and the pH range was 7.0-11.0 (optimum pH 8.0). The DNA G+C contents of strains T-11(T) and E-112(T) were 41.1 and 42.6 mol%, respectively. On the basis of 16S rRNA gene sequence similarity, the two strains were shown to be related most closely to Anoxybacillus species. The chemotaxonomic characteristics [predominant isoprenoid quinone menaquinone 7 (MK-7); major fatty acids iso-C(15 : 0) and iso-C(17 : 0)] also supported the affiliation of strains T-11(T) and E-112(T) to the genus Anoxybacillus. The results of DNA-DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strains T-11(T) and E-112(T) from Anoxybacillus species with validly published names. Strains T-11(T) and E-112(T) therefore represent two novel species, for which the names Anoxybacillus tengchongensis sp. nov. (type strain T-11(T) =CCTCC AB209237(T) =KCTC 13721(T)) and Anoxybacillus eryuanensis sp. nov. (type strain E-112(T) =CCTCC AB209236(T) =KCTC 13720(T)) are proposed.
Ann file
T1 Species 0 28 Anoxybacillus tengchongensis
N1 Reference T1 Taxonomy:576944
T2 Species 42 67 Anoxybacillus eryuanensis
N2 Reference T2 Taxonomy:651866
T3 Species 204 208 T-11
N3 Reference T3 Taxonomy:576944
T4 Species 216 221 E-112
N4 Reference T4 Taxonomy:651866
T5 Species 421 425 T-11
N5 Reference T5 Taxonomy:576944
T6 Species 553 558 E-112
N6 Reference T6 Taxonomy:651866
T7 Species 673 677 T-11
N7 Reference T7 Taxonomy:576944
T8 Species 685 690 E-112
N8 Reference T8 Taxonomy:651866
T9 Species 1046 1050 T-11
N9 Reference T9 Taxonomy:576944
T10 Species 1058 1063 E-112
N10 Reference T10 Taxonomy:651866
T11 Species 1232 1236 T-11
N11 Reference T11 Taxonomy:576944
T12 Species 1244 1249 E-112
N12 Reference T12 Taxonomy:651866
T13 Species 1318 1322 T-11
N13 Reference T13 Taxonomy:576944
T14 Species 1330 1335 E-112
N14 Reference T14 Taxonomy:651866
T15 Species 1398 1426 Anoxybacillus tengchongensis
N15 Reference T15 Taxonomy:576944
T16 Species 1449 1453 T-11
N16 Reference T16 Taxonomy:576944
T17 Species 1496 1521 Anoxybacillus eryuanensis
N17 Reference T17 Taxonomy:651866
T18 Species 1544 1549 E-112
N18 Reference T18 Taxonomy:651866