20637818
not annotated - annotated - LINNAEUS only
The Candida albicans Rgd1 is a RhoGAP protein involved in the control of filamentous growth.
Rho proteins are essential regulators of polarized growth in eukaryotic cells. These proteins are down-regulated in vivo by specific Rho GTPase Activating Proteins (RhoGAP). We investigated the role of Rgd1 RhoGAP, encoded by the Candida albicans RGD1 gene. We demonstrated that CaCdc42, CaRho3 and CaRho4 proteins had an intrinsic GTPase activity and that CaRgd1 stimulates in vitro GTP hydrolysis of these GTPases. Deletion of RGD1 in C. albicans results in sensitivity to low pH as already described for rgd1Delta in Saccharomyces cerevisiae. The role of Rgd1 in survival at low pH is conserved in the two yeast species as the CaRGD1 gene complements the Scrgd1Delta sensitivity. By tagging the RhoGAP with GFP, we found that CaRgd1 is localized at the tip and cortex of growing cells and during cytokinesis at the septation sites in yeast and filamentous forms. We investigated the effect of CaRgd1 on the control of the polarized growth. Removing CaRGD1 alleles increased filamentous growth and cells lacking CaRgd1 presented longer germ tubes. Conversely, RGD1 overexpression restricted hyphae growth. Our results demonstrate that Rgd1 is critical for filamentous formation in C. albicans especially for filamentous elongation.
Ann file
T1 Species 4 20 Candida albicans
N1 Reference T1 Taxonomy:5476
T2 Species 325 341 Candida albicans
N2 Reference T2 Taxonomy:5476
T3 Species 532 543 C. albicans
N3 Reference T3 Taxonomy:5476
T4 Species 615 639 Saccharomyces cerevisiae
N4 Reference T4 Taxonomy:4932
T5 Species 1278 1289 C. albicans
N5 Reference T5 Taxonomy:5476
T6 Out-of-scope 704 709 yeast
T7 Out-of-scope 932 937 yeast
N7 Reference T7 Taxonomy:147537 Saccharomycotina
N6 Reference T6 Taxonomy:147537 Saccharomycotina