20663714
not annotated - annotated - LINNAEUS only
Identification of a mastigoneme protein from Phytophthora nicotianae.
Tripartite tubular hairs (mastigonemes) on the anterior flagellum of protists in the stramenopile taxon are responsible for reversing the thrust of flagellar beat and for cell motility. Immunoprecipitation experiments using antibodies directed towards mastigonemes on the flagella of zoospores of Phytophthora nicotianae have facilitated the cloning of a gene encoding a mastigoneme shaft protein in this Oomycete. Expression of the gene, designated PnMas2, is up-regulated during asexual sporulation, a period during which many zoospore components are synthesized. Analysis of the sequence of the PnMas2 protein has revealed that, like other stramenopile mastigoneme proteins, PnMas2 has an N-terminal secretion signal and contains four cysteine-rich epidermal growth factor (EGF)-like domains. Evidence from non-denaturing gels indicates that PnMas2 forms large oligomeric complexes, most likely through disulphide bridging. Bioinformatic analysis has revealed that Phytophthora species typically contain three or four putative mastigoneme proteins containing the four EGF-like domains. These proteins are similar in sequence to mastigoneme proteins in other stramenopile protists including the algae Ochromonas danica, Aureococcus anophagefferens and Scytosiphon lomentaria and the diatoms Thalassiosira pseudonana and T. weissflogii.
Ann file
T1 Species 45 68 Phytophthora nicotianae
N1 Reference T1 Taxonomy:4790
T2 Species 368 391 Phytophthora nicotianae
N2 Reference T2 Taxonomy:4790
T3 Species 1274 1291 Ochromonas danica
N3 Reference T3 Taxonomy:2986
T4 Species 1293 1320 Aureococcus anophagefferens
N4 Reference T4 Taxonomy:44056
T5 Species 1325 1347 Scytosiphon lomentaria
N5 Reference T5 Taxonomy:27967
T6 Species 1364 1388 Thalassiosira pseudonana
N6 Reference T6 Taxonomy:35128
T7 Species 1393 1407 T. weissflogii
N7 Reference T7 Taxonomy:67004