20708435
not annotated - annotated - LINNAEUS only
Barcoding Tetrahymena: discriminating species and identifying unknowns using the cytochrome c oxidase subunit I (cox-1) barcode.
DNA barcoding using the mitochondrial cytochromecoxidase subunit I (cox-1) gene has recently gained popularity as a tool for species identification of a variety of taxa. The primary objective of our research was to explore the efficacy of using cox-1 barcoding for species identification within the genus Tetrahymena. We first increased intraspecific sampling for Tetrahymena canadensis, Tetrahymena hegewischi, Tetrahymena pyriformis, Tetrahymena rostrata, Tetrahymena thermophila, and Tetrahymena tropicalis. Increased sampling efforts show that intraspecific sequence divergence is typically less than 1%, though it may be more in some species. The barcoding also showed that some strains might be misidentified or mislabeled. We also used cox-1 barcodes to provide species identifications for 51 unidentified environmental isolates, with a success rate of 98%. Thus, cox-1 barcoding is an invaluable tool for protistologists, especially when used in conjunction with morphological studies.
Ann file
T1 Species 495 517 Tetrahymena canadensis
N1 Reference T1 Taxonomy:5894
T2 Species 519 541 Tetrahymena hegewischi
N2 Reference T2 Taxonomy:5923
T3 Species 543 565 Tetrahymena pyriformis
N3 Reference T3 Taxonomy:5908
T4 Species 567 587 Tetrahymena rostrata
N4 Reference T4 Taxonomy:5909
T5 Species 589 612 Tetrahymena thermophila
N5 Reference T5 Taxonomy:5911
T6 Species 618 640 Tetrahymena tropicalis
N6 Reference T6 Taxonomy:5912