20719250
not annotated - annotated - LINNAEUS only
Regulation of virulence factors, carbon utilization and virulence by SNF1 in Cryptococcus neoformans JEC21 and divergent actions of SNF1 between cryptococcal strains.
We describe here the functions of a Snf1/AMPK homolog in the human pathogenic yeast Cryptococcus neoformans, strain JEC21. We found that JEC21 SNF1 is a key regulator for the biosynthesis of the major virulence factors, stress resistance and alternative carbon source utilization. Disruption of JEC21 SNF1 results in defects of laccase activity and capsule production, sensitivity to cation stress. Especially, we found that JEC21 SNF1 is essential for growth at elevated temperature and for thermotolerance. To our knowledge, a role for Snf1 proteins in thermotolerance has not been reported. Furthermore, we observed a functional divergence between JEC21 SNF1 and its equivalent from serotype A strain H99. A high temperature is needed for H99 SNF1 to function in stress response and carbon source preference, but not for the JEC21 SNF1. Our results confirmed a critical role of JEC21 SNF1 in regulation of stress response and virulence. Revelation of divergent actions of SNF1 may help to understand the evolution of cryptococcal pathogenesis and provides insights into the strain-associated biosynthesis of virulence factors.
Ann file
T1 Species 77 106 Cryptococcus neoformans JEC21
N1 Reference T1 Taxonomy:5207
T2 Species 285 290 JEC21
N2 Reference T2 Taxonomy:5207
T3 Species 306 311 JEC21
N3 Reference T3 Taxonomy:5207
T4 Species 464 469 JEC21
N4 Reference T4 Taxonomy:5207
T5 Species 594 599 JEC21
N5 Reference T5 Taxonomy:5207
T6 Species 873 876 H99
N6 Reference T6 Taxonomy:5207
T7 Species 911 914 H99
N7 Reference T7 Taxonomy:5207
T8 Species 997 1002 JEC21
N8 Reference T8 Taxonomy:5207
T9 Species 1050 1055 JEC21
N9 Reference T9 Taxonomy:5207
T10 Species 253 276 Cryptococcus neoformans
T11 Species 230 235 human
T12 Out-of-scope 247 252 yeast
T13 Species 820 825 JEC21
N10 Reference T13 Taxonomy:5207
N11 Reference T10 Taxonomy:5207 Cryptococcus neoformans