20719251
not annotated - annotated - LINNAEUS only
Diversification and evolution of the avirulence gene AVR-Pita1 in field isolates of Magnaporthe oryzae.
Rice blast disease is the single most destructive plant disease that threatens stable rice production worldwide. Race-specific resistance to the rice blast pathogen has not been durable and the mechanism by which the resistance is overcome remains largely unknown. Here we report the molecular mechanisms of diversification and the instability of the avirulence gene AVR-Pita1 in field strains of Magnaporthe oryzae interacting with the host resistance gene Pi-ta and triggering race-specific resistance. Two-base-pair insertions resulting in frame-shift mutations and partial and complete deletions of AVR-Pita1 were identified in virulent isolates. Moreover, a total of 38 AVR-Pita1 haplotypes encoding 27 AVR-Pita1 variants were identified among 151 avirulent isolates. Most DNA sequence variation was found to occur in the exon region resulting in amino acid substitution. These findings demonstrate that AVR-Pita1 is under positive selection and mutations of AVR-Pita1 are responsible for defeating race-specific resistance in nature.
Ann file
T1 Species 84 102 Magnaporthe oryzae
N1 Reference T1 Taxonomy:318829
T2 Species 192 196 rice
N2 Reference T2 Taxonomy:4530
T3 Species 503 521 Magnaporthe oryzae
N3 Reference T3 Taxonomy:318829
T4 Species 106 110 Rice
T5 Species 251 270 rice blast pathogen
N4 Reference T5 Taxonomy:318829 Pyricularia oryzae