20807375
not annotated - annotated - LINNAEUS only
OsEDR1 negatively regulates rice bacterial resistance via activation of ethylene biosynthesis.
Rice OsEDR1 is a sequence ortholog of Arabidopsis EDR1. However, its molecular function is unknown. We show here that OsEDR1-suppressing/knockout (KO) plants, which developed spontaneous lesions on the leaves, have enhanced resistance to Xanthomonas oryzae pv. oryzae (Xoo) causing bacterial blight disease. This resistance was associated with increased accumulation of salicylic acid (SA) and jasmonic acid (JA), induced expression of SA- and JA-related genes and suppressed accumulation of 1-aminocyclopropane-1-carboxylic acid (ACC), the direct precursor of ethylene, and expression of ethylene-related genes. OsEDR1-KO plants also showed suppressed production of ethylene. Knockout of OsEDR1 suppressed the ACC synthase (ACS) gene family, which encodes the rate-limiting enzymes of ethylene biosynthesis by catalysing the formation of ACC. The lesion phenotype and enhanced bacterial resistance of the OsEDR1-KO plants was partly complemented by the treatment with ACC. ACC treatment was associated with decreased SA and JA biosynthesis in OsEDR1-KO plants. In contrast, aminoethoxyvinylglycine, the inhibitor of ethylene biosynthesis, promoted expression of SA and JA synthesis-related genes in OsEDR1-KO plants. These results suggest that ethylene is a negative signalling molecule in rice bacterial resistance. In the rice-Xoo interaction, OsEDR1 transcriptionally promotes the synthesis of ethylene that, in turn, suppresses SA- and JA-associated defence signalling.
Ann file
T1 Species 28 32 rice
N1 Reference T1 Taxonomy:4530
T2 Species 97 101 Rice
N2 Reference T2 Taxonomy:4530
T3 Species 335 364 Xanthomonas oryzae pv. oryzae
N3 Reference T3 Taxonomy:347
T4 Species 366 369 Xoo
N4 Reference T4 Taxonomy:347
T5 Species 1388 1392 rice
N5 Reference T5 Taxonomy:4530
T6 Species 1422 1426 rice
N6 Reference T6 Taxonomy:4530
T7 Species 1427 1430 Xoo
N7 Reference T7 Taxonomy:347
T8 Out-of-scope 135 146 Arabidopsis
N8 Reference T8 Taxonomy:3701 Arabidopsis