20817115
not annotated - annotated - LINNAEUS only
Characterization of the developmental regulator FlbE in Aspergillus fumigatus and Aspergillus nidulans.
Several upstream developmental activators control asexual development (conidiation) in Aspergillus. In this study, we characterize one of such activators called flbE in Aspergillus fumigatus and Aspergillus nidulans. The predicted FlbE protein is composed of 222 and 201 aa in A. fumigatus and A. nidulans, respectively. While flbE is transiently expressed during early phase of growth in A. nidulans, it is somewhat constitutively expressed during the lifecycle of A. fumigatus. The deletion of flbE causes reduced conidiation and delayed expression of brlA and vosA in both species. Moreover, FlbE is necessary for salt-induced development in liquid submerged culture in A. fumigatus. The A. nidulans flbE null mutation is fully complemented by A. fumigatus flbE, indicating a functional conservancy of FlbE in Aspergillus. Both the deletion and overexpression of flbE in A. nidulans result in developmental defects, enhanced autolysis, precocious cell death, and delayed expression of brlA/vosA, suggesting that balanced activity of FlbE is crucial for proper growth and development. Importantly, the N-terminal portion of FlbE exhibits the trans-activation ability in yeast, whereas the C-terminal half negatively affects its activity. Site-directed mutagenesis of certain conserved N-terminal amino acids abolishes the ability of trans-activation, overexpression-induced autolysis, and complementing the null mutation. Finally, overexpression of flbD, but not flbB or flbC, restores conidiation in A. nidulans DeltaflbE, generally supporting the current genetic model for developmental regulation.
Ann file
T1 Species 56 77 Aspergillus fumigatus
N1 Reference T1 Taxonomy:746128
T2 Species 82 102 Aspergillus nidulans
N2 Reference T2 Taxonomy:162425
T3 Species 275 296 Aspergillus fumigatus
N3 Reference T3 Taxonomy:746128
T4 Species 301 321 Aspergillus nidulans
N4 Reference T4 Taxonomy:162425
T5 Species 383 395 A. fumigatus
N5 Reference T5 Taxonomy:746128
T6 Species 400 411 A. nidulans
N6 Reference T6 Taxonomy:162425
T7 Species 495 506 A. nidulans
N7 Reference T7 Taxonomy:162425
T8 Species 572 584 A. fumigatus
N8 Reference T8 Taxonomy:746128
T9 Species 779 791 A. fumigatus
N9 Reference T9 Taxonomy:746128
T10 Species 797 808 A. nidulans
N10 Reference T10 Taxonomy:162425
T11 Species 853 865 A. fumigatus
N11 Reference T11 Taxonomy:746128
T12 Species 980 991 A. nidulans
N12 Reference T12 Taxonomy:162425
T13 Out-of-scope 1278 1283 yeast
T14 Species 1609 1620 A. nidulans
N14 Reference T14 Taxonomy:162425
N13 Reference T13 Taxonomy:147537 Saccharomycotina