20825577
not annotated - annotated - LINNAEUS only
OsRAN2, essential for mitosis, enhances cold tolerance in rice by promoting export of intranuclear tubulin and maintaining cell division under cold stress.
With global climate change, abnormally low temperatures have affected the world's rice production. Many genes have been shown to be essential for molecular improvement of rice cold-tolerance traits. However, less is known about the molecular cellular mechanism of their response to cold stress. Here, we investigated OsRAN2 involved in regulation of cell division during cold stress in rice. Expression of OsRAN2 was increased under cold treatment, but not during salt and drought stress. The mean root mitotic index was closely related to the expression level of OsRAN2. Knockdown transgenic rice lines showed an aberrant organization of spindles during mitosis and stunted growth during development. Overexpression of OsRAN2 enhanced cold tolerance in rice. The transgenic rice overexpressing OsRAN2 showed maintained cell division, decreased proportion of cells with intranuclear tubulin and formation of a normal nuclear envelope under the cold condition. Our study suggests a mechanism for OsRAN2 in regulating cold resistance in rice by maintaining cell division through promoting the normal export of intranuclear tubulin at the end of mitosis. This insight could help improve the cold-tolerance trait in rice.
Ann file
T1 Species 58 62 rice
N1 Reference T1 Taxonomy:4530
T2 Species 240 244 rice
N2 Reference T2 Taxonomy:4530
T3 Species 329 333 rice
N3 Reference T3 Taxonomy:4530
T4 Species 544 548 rice
N4 Reference T4 Taxonomy:4530
T5 Species 751 755 rice
N5 Reference T5 Taxonomy:4530
T6 Species 912 916 rice
N6 Reference T6 Taxonomy:4530
T7 Species 933 937 rice
N7 Reference T7 Taxonomy:4530
T8 Species 1193 1197 rice
N8 Reference T8 Taxonomy:4530
T9 Species 1370 1374 rice
N9 Reference T9 Taxonomy:4530