20850479
not annotated - annotated - LINNAEUS only
Identification of the structural proteins of VP1 and VP2 of a novel mud crab dicistrovirus.
Mud crab dicistrovirus (MCDV), a newly identified single-stranded positive RNA virus, is an important pathogen that causes serious economic losses to mud crab aquaculture. In this study, MCDV was purified, and three structural proteins of MCDV were separated by SDS-PAGE. The N-terminal 15 amino acids were sequenced and aligned with the main structural proteins of other dicistrovirus. The three structural proteins were named VP1, VP2 and VP3. Monoclonal antibodies (MAbs) against the two main structural proteins, VP1 and VP2, were prepared, and the two structural proteins were then identified using these MAbs. The results of Western blot analyses demonstrated that five MAbs recognised VP1 and two recognised VP2. The results of immunogold transmission electron microscopy (IEM) revealed that the epitopes of the two structural proteins recognised by the MAbs were located at the outer surface of the virions, which suggested that the two structural proteins are MCDV capsid proteins. The identification of the two structural proteins of MCDV is useful for studying their functions, as well as the mechanism of infection and the pathogenesis of MCDV.
Ann file
T1 Species 68 90 mud crab dicistrovirus
N1 Reference T1 Taxonomy:932662
T2 Species 94 116 Mud crab dicistrovirus
N2 Reference T2 Taxonomy:932662
T3 Species 118 122 MCDV
N3 Reference T3 Taxonomy:932662
T4 Species 244 252 mud crab
N4 Reference T4 Taxonomy:6761
T5 Species 281 285 MCDV
N5 Reference T5 Taxonomy:932662
T6 Species 333 337 MCDV
N6 Reference T6 Taxonomy:932662
T7 Species 1063 1067 MCDV
N7 Reference T7 Taxonomy:932662
T8 Species 1138 1142 MCDV
N8 Reference T8 Taxonomy:932662
T9 Species 1245 1249 MCDV
N9 Reference T9 Taxonomy:932662