20854394
not annotated - annotated - LINNAEUS only
SGT1 contributes to coronatine signaling and Pseudomonas syringae pv. tomato disease symptom development in tomato and Arabidopsis.
* Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) causes an economically important bacterial speck disease on tomato and produces symptoms with necrotic lesions surrounded by chlorosis. The chlorosis is mainly attributed to a jasmonic acid (JA)-isoleucine analogue, coronatine (COR), produced by Pst DC3000. However, the molecular processes underlying lesion development and COR-induced chlorosis are poorly understood. * In this study, we took advantage of a chlorotic phenotype elicited by COR on Nicotiana benthamiana leaves and virus-induced gene silencing (VIGS) as a rapid reverse genetic screening tool and identified a role for SGT1 (suppressor of G2 allele of skp1) in COR-induced chlorosis. * Silencing of SGT1 in tomato resulted in reduction of disease-associated symptoms (cell death and chlorosis), suggesting a molecular connection between COR-induced chlorosis and cell death. In Arabidopsis, AtSGT1b but not AtSGT1a was required for COR responses, including root growth inhibition and Pst DC3000 symptom (water soaked lesion) development. Notably, overexpression of AtSGT1b did not alter Pst DC3000 symptoms or sensitivity to COR. * Taken together, our results demonstrate that SGT1/SGT1b is required for COR-induced chlorosis and subsequent necrotic disease development in tomato and Arabidopsis. SGT1 is therefore a component of the COR/JA-mediated signal transduction pathway.
Ann file
T1 Species 45 76 Pseudomonas syringae pv. tomato
N1 Reference T1 Taxonomy:317
T2 Species 108 114 tomato
N2 Reference T2 Taxonomy:4081
T3 Species 136 174 Pseudomonas syringae pv. tomato DC3000
N3 Reference T3 Taxonomy:317
T4 Species 176 186 Pst DC3000
N4 Reference T4 Taxonomy:317
T5 Species 248 254 tomato
N5 Reference T5 Taxonomy:4081
T6 Species 434 444 Pst DC3000
N6 Reference T6 Taxonomy:317
T7 Species 637 658 Nicotiana benthamiana
N7 Reference T7 Taxonomy:4100
T8 Species 862 868 tomato
N8 Reference T8 Taxonomy:4081
T9 Species 1139 1149 Pst DC3000
N9 Reference T9 Taxonomy:317
T10 Species 1242 1252 Pst DC3000
N10 Reference T10 Taxonomy:317
T11 Species 1428 1434 tomato
N11 Reference T11 Taxonomy:4081
T12 Out-of-scope 1033 1044 Arabidopsis
N12 Reference T12 Taxonomy:3701 Arabidopsis
T13 Out-of-scope 119 130 Arabidopsis
N13 Reference T13 Taxonomy:3701 Arabidopsis
T14 Out-of-scope 1439 1450 Arabidopsis
N14 Reference T14 Taxonomy:3701 Arabidopsis