20854480
not annotated - annotated - LINNAEUS only
Expression of diverse midgut serine proteinases in the sericigenous Lepidoptera Antheraea assamensis (Helfer) is influenced by choice of host plant species.
Antheraea assamensis is reared on various species of the Lauraceae family from north-east India for its distinctive cocoon silk. We demonstrate differential expression of digestive trypsin and chymotrypsins in larvae feeding on a primary host, Persea bombycina Kosterm., in comparison to larvae feeding on Litsea monopetala Roxb. using in vitro proteolytic assays, zymogram analyses with proteinase inhibitors, restriction digestion of RNA-PCR amplicons and quantitative real-time PCR (RT-PCR). Eight novel members of the serine proteinase gene family were identified, including an intron-spliced trypsin (AaPb4) and seven putative chymotrypsins (AaPb2, AaPb4, AaPb12, AaLm4, AaLm6, AaLm19 and AaLm29). Midgut transcript levels of the putative trypsin were higher in larvae fed P. bombycina whereas levels of transcripts encoding putative chymotrypsins were higher in larvae reared on L. monopetala. Complex, differential expression of sequence divergent midgut serine proteinases may reflect the ability of lepidopteran larvae to feed on different species of host plants. Possible implications of host plant choice on the digestive physiology of A. assamensis are discussed.
Ann file
T1 Species 80 100 Antheraea assamensis
N1 Reference T1 Taxonomy:91021
T2 Species 159 179 Antheraea assamensis
N2 Reference T2 Taxonomy:91021
T3 Species 403 419 Persea bombycina
N3 Reference T3 Taxonomy:466588
T4 Species 465 482 Litsea monopetala
N4 Reference T4 Taxonomy:344080
T5 Species 937 949 P. bombycina
N5 Reference T5 Taxonomy:466588
T6 Species 1044 1057 L. monopetala
N6 Reference T6 Taxonomy:344080
T7 Species 1306 1319 A. assamensis
N7 Reference T7 Taxonomy:91021