20854482
not annotated - annotated - LINNAEUS only
Differential transcription of two highly divergent gut-expressed Bm86 antigen gene homologues in the tick Rhipicephalus appendiculatus (Acari: Ixodida).
The transcriptional control of gene expression is not well documented in the Arthropoda. We describe transcriptional analysis of two exceptionally divergent homologues (Ra86) of the Bm86 gut antigen from Rhipicephalus appendiculatus. Bm86 forms the basis of a commercial vaccine for the control of Rhipicephalus (Boophilus) microplus. The R. appendiculatus Ra86 proteins contain 654 and 693 amino acids, with only 80% amino acid sequence identity. Reverse-transcription PCR of gut cDNA showed transcription of only one genotype in individual female ticks. PCR amplification of 3' untranslated sequences from genomic DNA indicated that both variants could be encoded within a single genome. When both variants were present, one of the two Ra86 genotypes was transcriptionally dominant.
Ann file
T1 Species 106 134 Rhipicephalus appendiculatus
N1 Reference T1 Taxonomy:34631
T2 Species 359 387 Rhipicephalus appendiculatus
N2 Reference T2 Taxonomy:34631
T3 Species 453 488 Rhipicephalus (Boophilus) microplus
N3 Reference T3 Taxonomy:6941
T4 Species 494 511 R. appendiculatus
N4 Reference T4 Taxonomy:34631