20863856
not annotated - annotated - LINNAEUS only
Expression of the C-terminal ORF2 protein of duck astrovirus for application in a serological test.
Duck astrovirus (DAstV) is an important pathogen causing duck viral hepatitis (DVH), a highly contagious and fatal disease in young ducklings. To provide an antigen for a diagnostic serum test, the C-terminus of DAstV ORF2 protein was expressed in Escherichia coli. Four positive and 30 negative sera were used to validate the purified ORF2 protein by developing an indirect enzyme-linked immunosorbent assay (ELISA). No cross-reactions were found against other duck pathogens, including duck hepatitis A virus, duck plague herpesvirus, duck reovirus, Newcastle disease virus, and Riemerella anatipestifer 12/19Riemerella anatipestifer 12/19 (63.2%) and 26/51 (51%) sera samples from two flocks of ducks that survived DAstV infections in commercial duck farms were positive for DAstV by this method, respectively. Interestingly, DAstV-specific antibodies were also detected in 12 (28.6%) of 42 sera samples from a different flock without DVH, indicating a wide distribution of subclinical infections caused by DAstV.
Ann file
T1 Species 45 60 duck astrovirus
T2 Species 102 117 Duck astrovirus
T3 Species 119 124 DAstV
T4 Species 350 366 Escherichia coli
N4 Reference T4 Taxonomy:562
T5 Species 590 612 duck hepatitis A virus
N5 Reference T5 Taxonomy:691956
T6 Species 614 637 duck plague herpesvirus
N6 Reference T6 Taxonomy:104388
T7 Species 639 652 duck reovirus
N7 Reference T7 Taxonomy:38170
T8 Species 654 677 Newcastle disease virus
N8 Reference T8 Taxonomy:11176
T9 Species 683 713 Riemerella anatipestifer 12/19
N9 Reference T9 Taxonomy:34085
T10 Species 683 707;726 731 Riemerella anatipestifer 26/51
N10 Reference T10 Taxonomy:34085
T11 Species 790 795 DAstV
T12 Species 850 855 DAstV
T13 Species 901 906 DAstV
T14 Species 1082 1087 DAstV
T15 Species 314 319 DAstV
N15 Reference T15 Taxonomy:905828
N1 Reference T1 Taxonomy:1239439 Avastrovirus 3
N2 Reference T2 Taxonomy:1239439 Avastrovirus 3
N3 Reference T3 Taxonomy:1239439 Avastrovirus 3
N11 Reference T11 Taxonomy:1239439 Avastrovirus 3
N12 Reference T12 Taxonomy:1239439 Avastrovirus 3
N13 Reference T13 Taxonomy:1239439 Avastrovirus 3
N14 Reference T14 Taxonomy:1239439 Avastrovirus 3