20880205
not annotated - annotated - LINNAEUS only
Factors required for the high CO2 specificity of the anaerobically induced maize GapC4 promoter in transgenic tobacco.
Flooding, a natural cause of anaerobiosis, is often accompanied by high CO(2) concentrations in the flood water. Plants need to respond to these environmental conditions. Strong anaerobic reporter gene activity in tobacco (Nicotiana tabacum) controlled by the glycolytic glyceraldehyde-3-phosphate dehydrogenase (GapC4) promoter from maize (Zea mays) depends on the presence of CO(2) and light. To identify factors required for CO(2) regulated gene expression, promoter deletions fused to the Beta-glucuronidase reporter gene were studied in transgenic tobacco. Deletion of a 40 bp fragment directly upstream of the TATA box leads to increased anaerobic reporter gene activity both, in the presence and absence of CO(2). This deletion does not affect light specific anaerobic expression. A positive correlation between increasing CO(2) concentrations and gene activity is observed. Electrophoretic mobility shift experiments indicate that tobacco nuclear extracts harbour proteins that bind to part of the 40 bp fragment. Database assisted as well as experimental analysis reveal a role for AP2/EREBP transcription factors for conferring the high CO(2) specificity to the GapC4 promoter in tobacco leaves. This work highlights the importance for plants to respond to high environmental CO(2) concentrations under anaerobic conditions.
Ann file
T1 Species 75 80 maize
N1 Reference T1 Taxonomy:4577
T2 Species 110 117 tobacco
N2 Reference T2 Taxonomy:4097
T3 Species 335 342 tobacco
N3 Reference T3 Taxonomy:4097
T4 Species 344 361 Nicotiana tabacum
N4 Reference T4 Taxonomy:4097
T5 Species 455 460 maize
N5 Reference T5 Taxonomy:4577
T6 Species 462 470 Zea mays
N6 Reference T6 Taxonomy:4577
T7 Species 674 681 tobacco
N7 Reference T7 Taxonomy:4097
T8 Species 1060 1067 tobacco
N8 Reference T8 Taxonomy:4097
T9 Species 1311 1318 tobacco
N9 Reference T9 Taxonomy:4097