20923689
not annotated - annotated - LINNAEUS only
Simultaneous virus-specific detection of the two cassava brown streak-associated viruses by RT-PCR reveals wide distribution in East Africa, mixed infections, and infections in Manihot glaziovii.
The expanding cassava brown streak disease (CBSD) epidemic in East Africa is caused by two ipomoviruses (genus Ipomovirus; Potyviridae), namely, Cassava brown streak virus (CBSV), and Ugandan cassava brown streak virus (UCBSV) that was described recently. A reverse transcription polymerase chain reaction (RT-PCR) based diagnostic method was developed in this study for simultaneous virus-specific detection of the two viruses. Results showed that CBSV and UCBSV are distributed widely in the highlands (> 1000 m above the sea level) of the Lake Victoria zone in Uganda and Tanzania and also in the Indian Ocean costal lowlands of Tanzania. Isolates of UCBSV from the Lake Victoria zone were placed to two phylogenetic clusters in accordance with their origin in Uganda or Tanzania, respectively. Mixed infections with CBSV and UCBSV were detected in many cassava plants in the areas surveyed. CBSV was also detected in the perennial species Manihot glaziovii (DNA-barcoded in this study) in Tanzania, which revealed the first virus reservoir other than cassava. The method for detection of CBSV and UCBSV described in this study has important applications for plant quarantine, resistance breeding of cassava, and studies on epidemiology and control of CBSD in East Africa.
Ann file
T1 Species 49 56 cassava
N1 Reference T1 Taxonomy:3983
T2 Species 177 194 Manihot glaziovii
N2 Reference T2 Taxonomy:478098
T3 Species 212 219 cassava
N3 Reference T3 Taxonomy:3983
T4 Species 343 369 Cassava brown streak virus
N4 Reference T4 Taxonomy:137758
T5 Species 371 375 CBSV
N5 Reference T5 Taxonomy:137758
T6 Species 382 416 Ugandan cassava brown streak virus
N6 Reference T6 Taxonomy:946046
T7 Species 418 423 UCBSV
N7 Reference T7 Taxonomy:946046
T8 Species 647 651 CBSV
N8 Reference T8 Taxonomy:137758
T9 Species 656 661 UCBSV
N9 Reference T9 Taxonomy:946046
T10 Species 852 857 UCBSV
N10 Reference T10 Taxonomy:946046
T11 Species 1018 1022 CBSV
N11 Reference T11 Taxonomy:137758
T12 Species 1027 1032 UCBSV
N12 Reference T12 Taxonomy:946046
T13 Species 1055 1062 cassava
N13 Reference T13 Taxonomy:3983
T14 Species 1093 1097 CBSV
N14 Reference T14 Taxonomy:137758
T15 Species 1141 1158 Manihot glaziovii
N15 Reference T15 Taxonomy:478098
T16 Species 1253 1260 cassava
N16 Reference T16 Taxonomy:3983
T17 Species 1290 1294 CBSV
N17 Reference T17 Taxonomy:137758
T18 Species 1299 1304 UCBSV
N18 Reference T18 Taxonomy:946046
T19 Species 1401 1408 cassava
N19 Reference T19 Taxonomy:3983