20933017
not annotated - annotated - LINNAEUS only
Adaptation of a Madin-Darby canine kidney cell line to suspension growth in serum-free media and comparison of its ability to produce avian influenza virus to Vero and BHK21 cell lines.
Madin-Darby canine kidney (MDCK) cells are currently considered for influenza vaccine manufacturing. A drawback of these cells is their anchorage dependent growth, which greatly complicates process scale-up. In this paper a novel MDCK cell line (MDCK-SFS) is described that grows efficiently in suspension and retained high expression levels of both alpha-2,6 and alpha-2,3 sialic acid receptors, which bind preferably to human and avian influenza viruses, respectively. The production of avian influenza virus by BHK21, Vero and MDCK-SFS cell lines was compared. Although BHK21 cells consisted of two populations, one of which lacks the alpha-2,3 receptor, they supported the replication of two influenza strains to high titres. However, BHK21 cells are generally not applicable for influenza production since they supported the replication of six further strains poorly. MDCK-SFS cells yielded the highest infectious virus titres and virus genome equivalent concentration for five of the eight influenza strains analyzed and the highest hemagglutination activity for all eight virus strains. Taken together with their suitability for suspension growth this makes the MDCK-SFS cell line potentially useful for large scale influenza virus production.
Ann file
T1 Species 28 34 canine
N1 Reference T1 Taxonomy:9612
T2 Species 200 206 canine
N2 Reference T2 Taxonomy:9612
T3 Out-of-scope 134 155 avian influenza virus
T4 Out-of-scope 620 643 avian influenza viruses
T5 Out-of-scope 677 698 avian influenza virus
T6 Out-of-scope 1411 1426 influenza virus
T7 Out-of-scope 610 615;626 643 human influenza viruses
N3 Reference T3 Taxonomy:11308 Orthomyxoviridae
N7 Reference T7 Taxonomy:11308 Orthomyxoviridae
N4 Reference T4 Taxonomy:11308 Orthomyxoviridae
N6 Reference T6 Taxonomy:11308 Orthomyxoviridae
N5 Reference T5 Taxonomy:11308 Orthomyxoviridae