20946131
not annotated - annotated - LINNAEUS only
Host plant secondary metabolite profiling shows a complex, strain-dependent response of maize to plant growth-promoting rhizobacteria of the genus Azospirillum.
Most Azospirillum plant growth-promoting rhizobacteria (PGPR) benefit plant growth through source effects related to free nitrogen fixation and/or phytohormone production, but little is known about their potential effects on plant physiology. These effects were assessed by comparing the early impacts of three Azospirillum inoculant strains on secondary metabolite profiles of two different maize (Zea mays) cultivars. After 10d of growth in nonsterile soil, maize methanolic extracts were analyzed by reverse-phase high-performance liquid chromatography (RP-HPLC) and secondary metabolites identified by liquid chromatography/mass spectrometry (LC/MS) and nuclear magnetic resonance (NMR). Seed inoculation resulted in increased shoot biomass (and also root biomass with one strain) of hybrid PR37Y15 but had no stimulatory effect on hybrid DK315. In parallel, Azospirillum inoculation led to major qualitative and quantitative modifications of the contents of secondary metabolites, especially benzoxazinoids, in the maize plants. These modifications depended on the PGPR strainxplant cultivar combination. Thus, Azospirillum inoculation resulted in early, strain-dependent modifications in the biosynthetic pathways of benzoxazine derivatives in maize in compatible interactions. This is the first study documenting a PGPR effect on plant secondary metabolite profiles, and suggests the establishment of complex interactions between Azospirillum PGPR and maize.
Ann file
T1 Species 88 93 maize
N1 Reference T1 Taxonomy:4577
T2 Species 555 560 maize
N2 Reference T2 Taxonomy:4577
T3 Species 562 570 Zea mays
N3 Reference T3 Taxonomy:4577
T4 Species 623 628 maize
N4 Reference T4 Taxonomy:4577
T5 Species 1183 1188 maize
N5 Reference T5 Taxonomy:4577
T6 Species 1413 1418 maize
N6 Reference T6 Taxonomy:4577
T7 Species 1622 1627 maize
N7 Reference T7 Taxonomy:4577