20950650
not annotated - annotated - LINNAEUS only
Rapid detection of drug-resistant mutations in hepatitis B virus by the PCR-Invader assay.
Early detection of resistant mutations of hepatitis B virus (HBV) is important for patients on nucleos(t)ide analog therapy. An assay based on the PCR-Invader technology was developed to detect resistant mutations with high sensitivity. The assay specifically detects mutations at codons 180, 181, 184, 202, 204, and 250 of the HBV polymerase reverse transcriptase domain. These mutations result in resistance to lamivudine and entecavir. In mixtures of plasmids containing wild-type and resistant mutants, fold-over-zero values for resistant mutations were detected in 2% of the total. Seventy-five serum samples from patients, whose treatment had been switched from lamivudine to entecavir, were examined by the PCR-Invader assay and direct sequencing. The PCR-Invader assay detected all resistant mutations that were detected by direct sequencing and even detected the presence of mutants that direct sequencing could not. Cloning sequencing confirmed those mutations found by the PCR-Invader assay and not by direct sequencing. The PCR-Invader assay is a useful tool for the early detection of drug-resistant mutations.
Ann file
T1 Species 47 64 hepatitis B virus
N1 Reference T1 Taxonomy:10407
T2 Species 135 152 hepatitis B virus
N2 Reference T2 Taxonomy:10407
T3 Species 154 157 HBV
N3 Reference T3 Taxonomy:10407
T4 Species 421 424 HBV
N4 Reference T4 Taxonomy:10407