20951165
not annotated - annotated - LINNAEUS only
The validation of a real-time RT-PCR assay which detects influenza A and types simultaneously for influenza A H1N1 (2009) and oseltamivir-resistant (H275Y) influenza A H1N1 (2009).
Influenza A H1N1 (2009) was declared by the World Health Organisation (WHO) as the first influenza pandemic of the 21st century. Rapid detection of influenza A and differentiation of influenza A H1N1 (2009) and seasonal influenza A is beneficial. In addition the rapid detection of antiviral resistant strains of influenza A H1N1 (2009) would be useful for clinicians to allow for change to an effective treatment at a much earlier stage if resistance is found. It was the aim of this study to develop a real-time RT-PCR that can detect all influenza A viruses and type simultaneously for influenza A H1N1 (2009) and oseltamivir resistant (H275Y) influenza A H1N1 (2009). This multiplex assay will allow laboratories to screen respiratory samples for all types of influenza A, influenza A H1N1 (2009) virus and oseltamivir resistant (H275Y) influenza A H1N1 (2009) virus in a rapid and cost effective format, ensuring that typing methods for seasonal and avian viruses are used on a smaller subset of samples. Since most virology laboratories already offer a molecular service for influenza A this assay could easily be implemented into most areas at little cost therefore increasing local access to resistance testing.
Ann file
T1 Species 98 114 influenza A H1N1
N1 Reference T1 Taxonomy:11320
T2 Species 724 743 influenza A viruses
N2 Reference T2 Taxonomy:11320
T3 Species 772 788 influenza A H1N1
N3 Reference T3 Taxonomy:11320
T4 Species 830 846 influenza A H1N1
N4 Reference T4 Taxonomy:11320
T5 Species 947 958 influenza A
N5 Reference T5 Taxonomy:11320
T6 Species 960 976 influenza A H1N1
N6 Reference T6 Taxonomy:11320
T7 Species 1024 1040 influenza A H1N1
N7 Reference T7 Taxonomy:11320
T8 Species 183 199 Influenza A H1N1
N8 Reference T8 Taxonomy:11320
T9 Species 366 382 influenza A H1N1
N9 Reference T9 Taxonomy:11320
T10 Species 496 512 influenza A H1N1
N10 Reference T10 Taxonomy:11320
T11 Species 156 172 influenza A H1N1
N11 Reference T11 Taxonomy:11320
#1 AnnotatorNotes T11 TODO check span: virus related
#2 AnnotatorNotes T4 TODO check span: virus related
#3 AnnotatorNotes T7 TODO check span: virus related
T12 Species 331 342 influenza A
N12 Reference T12 Taxonomy:11320
T13 Species 403 414 influenza A
N13 Reference T13 Taxonomy:11320
T14 Species 57 68 influenza A
N14 Reference T14 Taxonomy:11320
T15 Species 1264 1275 influenza A
N15 Reference T15 Taxonomy:11320