20951743
not annotated - annotated - LINNAEUS only
Development and validation of a lateral flow immunoassay using colloidal gold for the identification of serotype-specific foot-and-mouth disease virus O, A and Asia 1.
A lateral flow immunoassay (LFI) was developed to identify and diagnose foot-and-mouth disease virus (FMDV) serotypes Ofoot-and-mouth disease virus (FMDV) serotypesfoot-and-mouth disease virus (FMDV) serotypes O, A and Asia 1. Antibodies obtained from rabbits and guinea pigs immunized with cell-culture-adapted virus strains (O/CHA/99, A/GS/LX/66, Asia 1/CHN/05) and suckling-mouse adapted virus strains (O/AV99(L), A/AV88(L), Asia 1/YNBS/58) were used as capture antibodies. The diagnostic kit included three immunochromatographic strips of types O, A and Asia 1, and the type-specific results were confirmed by color on the test lines of the three strips. The LFI was evaluated using epithelial and vesicular samples (n=396) prepared from current and historical field samples (provide by the National Foot-and-Mouth Disease Reference Laboratory of China at Lanzhou Veterinary Research Institute). Negative samples (n=95) were collected from healthy animals. The diagnostic sensitivity of the LFI for FMDV serotypes OFMDV serotypesFMDV serotypes O, A and Asia 1 was 88.3% compared to 89.7% obtained by the reference method of indirect-sandwich ELISA. The sensitivity of the LFI for FMDV type Asia 1 was higher at 92.1% compared to 90.5% for the ELISA. The specificity of the LFI was 97.1% compared with 97.4%.
Ann file
T1 Species 122 152 foot-and-mouth disease virus O
N1 Reference T1 Taxonomy:12110
T2 Species 154 155 A
N2 Reference T2 Taxonomy:12110
T3 Species 160 166 Asia 1
N3 Reference T3 Taxonomy:12110
T6 Species 242 289 foot-and-mouth disease virus (FMDV) serotypes O
N6 Reference T6 Taxonomy:12110
T7 Species 242 287;291 292 foot-and-mouth disease virus (FMDV) serotypes A
N7 Reference T7 Taxonomy:12110
T8 Species 242 287;297 303 foot-and-mouth disease virus (FMDV) serotypes Asia 1
N8 Reference T8 Taxonomy:12110
T9 Species 330 337 rabbits
N9 Reference T9 Taxonomy:9986
T10 Species 405 413 O/CHA/99
N10 Reference T10 Taxonomy:12110
T11 Species 415 425 A/GS/LX/66
N11 Reference T11 Taxonomy:12110
T12 Species 427 440 Asia 1/CHN/05
N12 Reference T12 Taxonomy:12110
T13 Species 455 460 mouse
N13 Reference T13 Taxonomy:10090
T14 Species 484 493 O/AV99(L)
N14 Reference T14 Taxonomy:12110
T15 Species 495 504 A/AV88(L)
N15 Reference T15 Taxonomy:12110
T16 Species 506 520 Asia 1/YNBS/58
N16 Reference T16 Taxonomy:12110
T17 Species 627 628 O
N17 Reference T17 Taxonomy:12110
T18 Species 630 631 A
N18 Reference T18 Taxonomy:12110
T19 Species 636 642 Asia 1
N19 Reference T19 Taxonomy:12110
T20 Species 1081 1097 FMDV serotypes O
N20 Reference T20 Taxonomy:12110
T22 Species 1081 1095;1099 1100 FMDV serotypes A
N22 Reference T22 Taxonomy:12110
T23 Species 1081 1095;1105 1111 FMDV serotypes Asia 1
N23 Reference T23 Taxonomy:12110
T24 Species 1232 1248 FMDV type Asia 1
N24 Reference T24 Taxonomy:12110
T21 Species 342 353 guinea pigs
N21 Reference T21 Taxonomy:10141 Cavia porcellus