20951744
not annotated - annotated - LINNAEUS only
Implementation and validation of a sensitive PCR detection method in the eradication campaign against Aleutian mink disease virus.
Aleutian mink disease virus (AMDV) is a severe progressive disease causing multiple different clinical syndromes in mink. In Denmark, the disease is notifiable and under official control. The control programme, based on serological screening, has confined successfully AMDV to the northern part of Denmark. However, re-infections and new introductions of virus into farms require a confirmatory virological test to verify the positive test results of single animals and ultimately to investigate disease transmission. A one step PCR amplifying a 374-base fragment of the NS1 gene of AMDV was compared to the counter-current immune electrophoresis (CIE) routinely used in the serological screening programme. Mink organs (n=299) obtained from 55 recently infected farms and 8 non-infected farms from 2008 to 2010 were tested by PCR, and the results were found to have a high correlation with the serological status of the mink. The relative diagnostic sensitivity of the PCR was 94.7%, and the relative diagnostic specificity was 97.9% when read in parallel with the CIE. PCR positive samples were sequenced and phylogenetic analysis revealed high similarity within the analysed AMDV strains and to AMDV strains described previously.
Ann file
T1 Species 102 129 Aleutian mink disease virus
N1 Reference T1 Taxonomy:28314
T2 Species 133 160 Aleutian mink disease virus
N2 Reference T2 Taxonomy:28314
T3 Species 249 253 mink
N3 Reference T3 Taxonomy:452646
T4 Species 402 406 AMDV
N4 Reference T4 Taxonomy:28314
T5 Species 716 720 AMDV
N5 Reference T5 Taxonomy:28314
T6 Species 841 845 Mink
N6 Reference T6 Taxonomy:452646
T7 Species 1054 1058 mink
N7 Reference T7 Taxonomy:452646
T8 Species 1311 1315 AMDV
N8 Reference T8 Taxonomy:28314
T9 Species 1331 1335 AMDV
N9 Reference T9 Taxonomy:28314
T10 Species 162 166 AMDV
N10 Reference T10 Taxonomy:28314