20958806
not annotated - annotated - LINNAEUS only
Neuropeptide precursor gene discovery in the Chagas disease vector Rhodnius prolixus.
We show a straightforward workflow combining homology search in Rhodnius prolixus genome sequence with cloning by rapid amplification of cDNA ends and mass spectrometry. We have identified 32 genes and their transcripts that encode a number of neuropeptide precursors leading to 194 putative peptides. We validated by mass spectrometry 82 of those predicted neuropeptides in the brain of R. prolixus to achieve the first comprehensive genomic, transcriptomic and neuropeptidomic analysis of an insect disease vector. Comparisons of available insect neuropeptide sequences revealed that the R. prolixus genome contains most of the conserved neuropeptides in insects, many of them displaying specific features at the sequence level. Some gene families reported here are identified for the first time in the order Hemiptera, a highly biodiverse group of insects that includes many human, animal and plant disease agents.
Ann file
T1 Species 67 84 Rhodnius prolixus
N1 Reference T1 Taxonomy:13249
T2 Species 152 169 Rhodnius prolixus
N2 Reference T2 Taxonomy:13249
T3 Species 476 487 R. prolixus
N3 Reference T3 Taxonomy:13249
T4 Species 678 689 R. prolixus
N4 Reference T4 Taxonomy:13249
T5 Species 966 971 human
N5 Reference T5 Taxonomy:9606