20958808
not annotated - annotated - LINNAEUS only
The RNA-Seq approach to studying the expression of mosquito mitochondrial genes.
In this study, we used extensive expressed sequence tag evidence obtained through 454 and Solexa next-generation sequencing to explore mtDNA transcription in male and female first instar larvae of Aedes aegypti and adults of Aedes aegypti, Anopheles gambiae, and Anopheles quadrimaculatus. Relative abundances of individual transcripts differed considerably within each sample, consistent with the differential stability of messenger RNA species. Large differences were also observed between species and between larval and adult stages; however, the male and female larval samples were remarkably similar. Quantitative PCR analysis of selected genes, cox1, l-rRNA and nd5, in larvae and adults of Ae. aegypti and in An. gambiae adults was consistent with the RNA-Seq-based quantification of expression. Finally, the absence of a conserved mtDNA region involved in transcriptional control in other dipterans suggests that mosquitoes have evolved a distinct mechanism of regulation of gene expression in the mitochondrion.
Ann file
T1 Species 280 293 Aedes aegypti
N1 Reference T1 Taxonomy:7159
T2 Species 308 321 Aedes aegypti
N2 Reference T2 Taxonomy:7159
T3 Species 323 340 Anopheles gambiae
N3 Reference T3 Taxonomy:7165
T4 Species 346 371 Anopheles quadrimaculatus
N4 Reference T4 Taxonomy:7166
T5 Species 780 791 Ae. aegypti
N5 Reference T5 Taxonomy:7159
T6 Species 799 810 An. gambiae
N6 Reference T6 Taxonomy:7165