20961294
not annotated - annotated - LINNAEUS only
Isolation and characterization of MAT genes in the symbiotic ascomycete Tuber melanosporum.
* The genome of Tuber melanosporum has recently been sequenced. Here, we used this information to identify genes involved in the reproductive processes of this edible fungus. The sequenced strain (Mel28) possesses only one of the two master genes required for mating, that is, the gene that codes for the high mobility group (HMG) transcription factor (MAT1-2-1), whereas it lacks the gene that codes for the protein containing the alpha-box- domain (MAT1-1-1), suggesting that this fungus is heterothallic. * A PCR-based approach was initially employed to screen truffles for the presence of the MAT1-2-1 gene and amplify the conserved regions flanking the mating type (MAT) locus. The MAT1-1-1 gene was finally identified using primers designed from the conserved regions of strains that lack the MAT1-2-1 gene. * Mating type-specific primer pairs were developed to screen asci and gleba from truffles of different origins and to genotype single ascospores within the asci. These analyses provided definitive evidence that T. melanosporum is a heterothallic species with a MAT locus that is organized similarly to those of ancient fungal lineages. * A greater understanding of the reproductive mechanisms that exist in Tuber spp. allows for optimization of truffle plantation management strategies.
Ann file
T1 Species 72 90 Tuber melanosporum
N1 Reference T1 Taxonomy:39416
T2 Species 110 128 Tuber melanosporum
N2 Reference T2 Taxonomy:39416
T3 Species 1119 1134 T. melanosporum
N3 Reference T3 Taxonomy:39416
T4 Species 658 666 truffles
N4 Reference T4 Taxonomy:39416 Tuber melanosporum
#1 AnnotatorNotes T4 TODO check
T5 Species 989 997 truffles
N5 Reference T5 Taxonomy:39416 Tuber melanosporum
#2 AnnotatorNotes T5 TODO check
T6 Species 1353 1360 truffle
N6 Reference T6 Taxonomy:39416 Tuber melanosporum
#3 AnnotatorNotes T6 TODO check