20962077
not annotated - annotated - LINNAEUS only
Simian immunodeficiency virus from the sooty mangabey and rhesus macaque is modified with O-linked carbohydrate.
Although stretches of serine and threonine are sometimes sites for O-linked carbohydrate attachment, specific sequence and structural determinants for O-linked attachment remain ill defined. The gp120 envelope protein of SIVmac239 contains a serine-threonine-rich stretch of amino acids at positions 128 to 139. Here we show that lectin protein from jackfruit seed (jacalin), which binds to non- and monosialylated core 1 O-linked carbohydrate, potently inhibited the replication of SIVmac239. Selection of a jacalin-resistant SIVmac239 variant population resulted in virus with specific substitutions within amino acids 128 to 139. Cloned simian immunodeficiency virus (SIV) variants with substitutions in the 128-to-139 region had infectivities equivalent to, or within 1 log unit of, that of SIVmac239 and were resistant to the inhibitory effects of jacalin. Characterization of the SIVmac239 gp120 O-linked glycome showed the presence of core 1 and core 2 O-linked carbohydrate; a 128-to-139-substituted variant gp120 from jacalin-resistant SIV lacked O-linked carbohydrate. Unlike that of SIVmac239, the replication of HIV-1 strain NL4-3 was resistant to inhibition by jacalin. Purified gp120s from four SIVmac and SIVsm strains bound jacalin strongly in an enzyme-linked immunosorbent assay, while nine different HIV-1 gp120s, two SIVcpz gp120s, and 128-to-139-substituted SIVmac239 gp120 did not bind jacalin. The ability or inability to bind jacalin thus correlated with the presence of the serine-threonine-rich stretch in the SIVmac and SIVsm gp120s and the absence of such stretches in the SIVcpz and HIV-1 gp120s. Consistent with sequence predictions, two HIV-2 gp120s bound jacalin, while one did not. These data demonstrate the presence of non- and monosialylated core 1 O-linked carbohydrate on the gp120s of SIVmac and SIVsm and the lack of these modifications on HIV-1 and SIVcpz gp120s.
Ann file
T1 Species 0 29 Simian immunodeficiency virus
N1 Reference T1 Taxonomy:11723
T2 Species 39 53 sooty mangabey
N2 Reference T2 Taxonomy:9531
T3 Species 58 72 rhesus macaque
N3 Reference T3 Taxonomy:9544
T4 Species 336 345 SIVmac239
N4 Reference T4 Taxonomy:11723
T5 Species 465 474 jackfruit
N5 Reference T5 Taxonomy:3489
T6 Species 598 607 SIVmac239
N6 Reference T6 Taxonomy:11723
T7 Species 642 651 SIVmac239
N7 Reference T7 Taxonomy:11723
T8 Species 755 784 simian immunodeficiency virus
N8 Reference T8 Taxonomy:11723
T9 Species 786 789 SIV
N9 Reference T9 Taxonomy:11723
T10 Species 910 919 SIVmac239
N10 Reference T10 Taxonomy:11723
T11 Species 1001 1010 SIVmac239
N11 Reference T11 Taxonomy:11723
T12 Species 1160 1163 SIV
N12 Reference T12 Taxonomy:11723
T13 Species 1209 1218 SIVmac239
N13 Reference T13 Taxonomy:11723
T14 Species 1239 1257 HIV-1 strain NL4-3
N14 Reference T14 Taxonomy:11676
T15 Species 1324 1330 SIVmac
N15 Reference T15 Taxonomy:11723
T16 Species 1335 1340 SIVsm
N16 Reference T16 Taxonomy:11723
T17 Species 1434 1439 HIV-1
N17 Reference T17 Taxonomy:11676
T18 Species 1452 1458 SIVcpz
N18 Reference T18 Taxonomy:11723
T19 Species 1494 1503 SIVmac239
N19 Reference T19 Taxonomy:11723
T20 Species 1651 1657 SIVmac
N20 Reference T20 Taxonomy:11723
T21 Species 1662 1667 SIVsm
N21 Reference T21 Taxonomy:11723
T22 Species 1716 1722 SIVcpz
N22 Reference T22 Taxonomy:11723
T23 Species 1727 1732 HIV-1
N23 Reference T23 Taxonomy:11676
T24 Species 1783 1788 HIV-2
N24 Reference T24 Taxonomy:11709
T25 Species 1939 1945 SIVmac
N25 Reference T25 Taxonomy:11723
T26 Species 1950 1955 SIVsm
N26 Reference T26 Taxonomy:11723
T27 Species 1995 2000 HIV-1
N27 Reference T27 Taxonomy:11676
T28 Species 2005 2011 SIVcpz
N28 Reference T28 Taxonomy:11723