20962080
not annotated - annotated - LINNAEUS only
Identification of binary interactions between human cytomegalovirus virion proteins.
Human cytomegalovirus (HCMV) virions are composed of a DNA-containing nucleocapsid surrounded by a tegument layer and host-derived lipid envelope studded with virally encoded glycoproteins. These complex virions are estimated to be composed of more than 50 viral proteins. Assembly of HCMV virions is poorly understood, especially with respect to acquisition of the tegument; however, it is thought to involve the stepwise addition of virion components through protein-protein interactions. We sought to identify interactions among HCMV virion proteins using yeast two-hybrid analysis. Using 33 known capsid and tegument proteins, we tested 1,089 pairwise combinations for binary interaction in the two-hybrid assay. We identified 24 interactions among HCMV virion proteins, including 13 novel interactions among tegument proteins and one novel interaction between capsid proteins. Several of these novel interactions were confirmed by coimmunoprecipitation of protein complexes from transfected cells. In addition, we demonstrate three of these interactions in the context of HCMV infection. This study reveals several new protein-protein interactions among HCMV tegument proteins, some of which are likely important for HCMV replication and pathogenesis.
Ann file
T1 Species 46 67 human cytomegalovirus
N1 Reference T1 Taxonomy:10359
T2 Species 87 108 Human cytomegalovirus
N2 Reference T2 Taxonomy:10359
T3 Species 110 114 HCMV
N3 Reference T3 Taxonomy:10359
T4 Species 372 376 HCMV
N4 Reference T4 Taxonomy:10359
T5 Species 619 623 HCMV
N5 Reference T5 Taxonomy:10359
T6 Out-of-scope 646 651 yeast
T7 Species 840 844 HCMV
N7 Reference T7 Taxonomy:10359
T8 Species 1164 1168 HCMV
N8 Reference T8 Taxonomy:10359
T9 Species 1246 1250 HCMV
N9 Reference T9 Taxonomy:10359
T10 Species 1309 1313 HCMV
N10 Reference T10 Taxonomy:10359
N6 Reference T6 Taxonomy:147537 Saccharomycotina