20962089
not annotated - annotated - LINNAEUS only
Antiviral effects of a transgenic RNA-dependent RNA polymerase.
Transgenic expression of the RNA-dependent RNA polymerase 3D(pol) inhibited infection of Theiler's murine encephalitis virus (TMEV), a picornavirus from which it was derived. Here, we infected 3D(pol) transgenic mice with another picornavirus, as well as an alphaherpesvirus and a rhabdovirus. 3D(pol) transgenic FVB mice had significantly lower viral loads and survived longer after infection with all three types of viruses than nontransgenic FVB mice. Viral inhibition among three different types of virus by transgenic 3D(pol) suggests that the mechanism of action is not the direct interference with picornaviral 3D(pol) but instead may be the changing of host cells to an antiviral state before or after viral infection occurs, as basal interferon levels were higher in 3D(pol) transgenic mice before infection. Further study of this mechanism may open new possibilities for future antiviral therapy.
Ann file
T1 Species 154 189 Theiler's murine encephalitis virus
N1 Reference T1 Taxonomy:204711
T2 Species 191 195 TMEV
N2 Reference T2 Taxonomy:204711
T3 Species 277 281 mice
N3 Reference T3 Taxonomy:10090
T4 Species 382 386 mice
N4 Reference T4 Taxonomy:10090
T5 Species 514 518 mice
N5 Reference T5 Taxonomy:10090
T6 Species 860 864 mice
N6 Reference T6 Taxonomy:10090