20962092
not annotated - annotated - LINNAEUS only
Induction of type I interferon secretion through recombinant Newcastle disease virus expressing measles virus hemagglutinin stimulates antibody secretion in the presence of maternal antibodies.
Measles virus (MV) vaccine effectively protects seronegative individuals against infection. However, inhibition of vaccine-induced seroconversion by maternal antibodies after vaccination remains a problem, as it leaves infants susceptible to MV infection. In cotton rats, passive transfer of MV-specific IgG mimics maternal antibodies and inhibits vaccine-induced seroconversion. Here, we report that immunization in the presence of passively transferred IgG inhibits the secretion of neutralizing antibodies but not the generation of MV-specific B cells. This finding suggested that MV-specific B cells require an additional stimulus to mature into antibody-secreting plasma cells. In order to provide such a stimulus, we generated a recombinant Newcastle disease virus (NDV) expressing the MV hemagglutinin (NDV-H). In contrast to MV, NDV-H induced high levels of type I interferon in plasmacytoid dendritic cells and in lung tissue. In cotton rats immunized with NDV-H, neutralizing antibodies were also generated in the presence of passively transferred antibodies. In the latter case, however, the level and kinetics of antibody generation were reduced. In vitro, alpha interferon stimulated the activation of MV-specific B cells from MV-immune spleen cells. NDV infection (which induces alpha interferon) had the same effect, and stimulation could be abrogated by antibodies neutralizing alpha interferon, but not interleukin 6 (IL-6). In vivo, coapplication of UV-inactivated MV with NDV led to increased MV-specific antibody production in the presence and absence of passively transferred antibodies. These data indicate that MV-specific B cells are being generated after immunization in the presence of maternal antibodies and that the provision of alpha interferon as an additional signal leads to antibody secretion.
Ann file
T1 Species 61 84 Newcastle disease virus
N1 Reference T1 Taxonomy:11176
T2 Species 96 109 measles virus
N2 Reference T2 Taxonomy:11234
T3 Species 196 209 Measles virus
N3 Reference T3 Taxonomy:11234
T4 Species 211 213 MV
N4 Reference T4 Taxonomy:11234
T5 Species 438 440 MV
N5 Reference T5 Taxonomy:11234
T6 Species 488 490 MV
N6 Reference T6 Taxonomy:11234
T7 Species 731 733 MV
N7 Reference T7 Taxonomy:11234
T8 Species 780 782 MV
N8 Reference T8 Taxonomy:11234
T9 Species 943 966 Newcastle disease virus
N9 Reference T9 Taxonomy:11176
T10 Species 968 971 NDV
N10 Reference T10 Taxonomy:11176
T11 Species 988 990 MV
N11 Reference T11 Taxonomy:11234
T12 Species 1006 1011 NDV-H
N12 Reference T12 Taxonomy:11176
T13 Species 1029 1031 MV
N13 Reference T13 Taxonomy:11234
T14 Species 1033 1038 NDV-H
N14 Reference T14 Taxonomy:11176
T15 Species 1162 1167 NDV-H
N15 Reference T15 Taxonomy:11176
T16 Species 1411 1413 MV
N16 Reference T16 Taxonomy:11234
T17 Species 1436 1438 MV
N17 Reference T17 Taxonomy:11234
T18 Species 1460 1463 NDV
N18 Reference T18 Taxonomy:11176
T19 Species 1679 1681 MV
N19 Reference T19 Taxonomy:11234
T20 Species 1687 1690 NDV
N20 Reference T20 Taxonomy:11176
T21 Species 1708 1710 MV
N21 Reference T21 Taxonomy:11234
T22 Species 1830 1832 MV
N22 Reference T22 Taxonomy:11234
#1 AnnotatorNotes T12 TODO check
T23 Species 455 466 cotton rats
N23 Reference T23 Taxonomy:42414 Sigmodon
T24 Species 1135 1146 cotton rats
N24 Reference T24 Taxonomy:42414 Sigmodon