20962095
not annotated - annotated - LINNAEUS only
Role of RNase MRP in viral RNA degradation and RNA recombination.
RNA degradation, together with RNA synthesis, controls the steady-state level of viral RNAs in infected cells. The endoribonucleolytic cleavage of viral RNA is important not only for viral RNA degradation but for RNA recombination as well, due to the participation of some RNA degradation products in the RNA recombination process. To identify host endoribonucleases involved in degradation of Tomato bushy stunt virus (TBSV) in a Saccharomyces cerevisiae model host, we tested eight known endoribonucleases. Here we report that downregulation of SNM1, encoding a component of the RNase MRP, and a temperature-sensitive mutation in the NME1 gene, coding for the RNA component of RNase MRP, lead to reduced production of the endoribonucleolytically cleaved TBSV RNA in yeast. We also show that the highly purified yeast RNase MRP cleaves the TBSV RNA in vitro, resulting in TBSV RNA degradation products similar in size to those observed in yeast cells. Knocking down the NME1 homolog in Nicotiana benthamiana also led to decreased production of the cleaved TBSV RNA, suggesting that in plants, RNase MRP is involved in TBSV RNA degradation. Altogether, this work suggests a role for the host endoribonuclease RNase MRP in viral RNA degradation and recombination.
Ann file
T1 Species 461 485 Tomato bushy stunt virus
N1 Reference T1 Taxonomy:12145
T2 Species 487 491 TBSV
N2 Reference T2 Taxonomy:12145
T3 Species 498 522 Saccharomyces cerevisiae
N3 Reference T3 Taxonomy:4932
T4 Species 823 827 TBSV
N4 Reference T4 Taxonomy:12145
T5 Out-of-scope 835 840 yeast
T6 Out-of-scope 880 885 yeast
T7 Species 908 912 TBSV
N7 Reference T7 Taxonomy:12145
T8 Species 940 944 TBSV
N8 Reference T8 Taxonomy:12145
T9 Out-of-scope 1007 1012 yeast
T10 Species 1054 1075 Nicotiana benthamiana
N10 Reference T10 Taxonomy:4100
T11 Species 1124 1128 TBSV
N11 Reference T11 Taxonomy:12145
T12 Species 1186 1190 TBSV
N12 Reference T12 Taxonomy:12145
N5 Reference T5 Taxonomy:147537 Saccharomycotina
N6 Reference T6 Taxonomy:147537 Saccharomycotina
N9 Reference T9 Taxonomy:147537 Saccharomycotina