20962099
not annotated - annotated - LINNAEUS only
Replicative and transcriptional activities of hepatitis B virus in patients coinfected with hepatitis B and hepatitis delta virusesviruses.
Hepatitis B virus (HBV) and hepatitis delta virus (HDV) interplay was investigated by examining liver and serum samples from 21 coinfected and 22 HBV-monoinfected patients with chronic liver disease. Different real-time PCR assays were applied to evaluate intrahepatic amounts of HBV DNA, covalently closed circular DNA (cccDNA), pregenomic RNA (pgRNA), pre-S/S RNAs, and HDV RNA. Besides HBV DNA and HDV RNA levels, HBsAg concentrations in the sera were also determined. HDV-coinfected cases showed significantly lower median levels of serum HBV DNA (-5 log), intrahepatic relaxed-circular DNA (-2 log), and cccDNA (-2 log) than those of HBV-monoinfected cases. Interestingly, pgRNA and pre-S/S RNA amounts were significantly lower (both -1 log) in HDV-positive patients, whereas serum HBsAg concentrations were comparable between the two patient groups. Pre-S/S RNA and HBsAg amounts per cccDNA molecule were higher in HDV-positive patients (3-fold and 1 log, respectively), showing that HBV replication was reduced, whereas synthesis of envelope proteins was not specifically decreased. The ratios of cccDNA to intracellular total HBV DNA showed a larger proportion of cccDNA molecules in HDV-positive cases. For these patients, both intrahepatic and serum HDV RNA amounts were associated with cccDNA but not with HBsAg or HBV DNA levels. Finally, HBV genomes with large deletions in the basal core promoter/precore region were detected in 5/21 HDV-positive patients but in no HDV-negative patients and were associated with lower viremia levels. These findings provide significant information about the interference exerted by HDV on HBV replication and transcription activities in the human liver.
Ann file
T1 Species 46 63 hepatitis B virus
N1 Reference T1 Taxonomy:10407
T2 Species 124 131;92 103 viruses hepatitis B
N2 Reference T2 Taxonomy:10407
T3 Species 108 131 hepatitis delta viruses
N3 Reference T3 Taxonomy:12475
T4 Species 135 152 Hepatitis B virus
N4 Reference T4 Taxonomy:10407
T5 Species 154 157 HBV
N5 Reference T5 Taxonomy:10407
T6 Species 163 184 hepatitis delta virus
N6 Reference T6 Taxonomy:12475
T7 Species 186 189 HDV
N7 Reference T7 Taxonomy:12475
T8 Species 281 284 HBV
N8 Reference T8 Taxonomy:10407
T9 Species 415 418 HBV
N9 Reference T9 Taxonomy:10407
T10 Species 507 510 HDV
N10 Reference T10 Taxonomy:12475
T11 Species 524 527 HBV
N11 Reference T11 Taxonomy:10407
T12 Species 536 539 HDV
N12 Reference T12 Taxonomy:12475
T13 Species 607 610 HDV
N13 Reference T13 Taxonomy:12475
T14 Species 678 681 HBV
N14 Reference T14 Taxonomy:10407
T15 Species 774 777 HBV
N15 Reference T15 Taxonomy:10407
T16 Species 885 888 HDV
N16 Reference T16 Taxonomy:12475
T17 Species 1056 1059 HDV
N17 Reference T17 Taxonomy:12475
T18 Species 1125 1128 HBV
N18 Reference T18 Taxonomy:10407
T19 Species 1269 1272 HBV
N19 Reference T19 Taxonomy:10407
T20 Species 1327 1330 HDV
N20 Reference T20 Taxonomy:12475
T21 Species 1395 1398 HDV
N21 Reference T21 Taxonomy:12475
T22 Species 1583 1586 HDV
N22 Reference T22 Taxonomy:12475
T23 Species 1615 1618 HDV
N23 Reference T23 Taxonomy:12475
T24 Species 1772 1775 HBV
N24 Reference T24 Taxonomy:10407
T25 Species 1824 1829 human
N25 Reference T25 Taxonomy:9606
T26 Species 1461 1464 HBV
N26 Reference T26 Taxonomy:10407
T27 Species 1486 1489 HBV
N27 Reference T27 Taxonomy:10407
T28 Species 1765 1768 HDV
N28 Reference T28 Taxonomy:12475