20964691
not annotated - annotated - LINNAEUS only
Tuber melanosporum: mating type distribution in a natural plantation and dynamics of strains of different mating types on the roots of nursery-inoculated host plants.
* In light of the recent finding that Tuber melanosporum, the ectomycorrhizal ascomycete that produces the most highly prized black truffles, is a heterothallic species, we monitored the spatial distribution of strains with opposite mating types (MAT) in a natural truffle ground and followed strain dynamics in artificially inoculated host plants grown under controlled conditions. * In a natural truffle ground, ectomycorrhizas (ECMs), soil samples and fruit bodies were sampled and genotyped to determine mating types. Simple sequence repeat (SSR) markers were also used to fingerprint ECMs and fruit bodies. The ECMs from nursery-inoculated host plants were analysed for mating type at 6 months and 19 months post-inoculation. * In open-field conditions, all ECMs from the same sampling site showed an identical mating type and an identical haploid genotype, based on SSR analysis. Interestingly, the gleba of fruit bodies always demonstrated the same genotype as the surrounding ECMs. Although root tips from nursery-grown plants initially developed ECMs of both mating types, a dominance of ECMs of the same MAT were found after several months. * The present study deepens our understanding of the vegetative and sexual propagation modes of T. melanosporum. These results are highly relevant for truffle cultivation.
Ann file
T1 Species 0 18 Tuber melanosporum
N1 Reference T1 Taxonomy:39416
T2 Species 207 225 Tuber melanosporum
N2 Reference T2 Taxonomy:39416
T3 Species 295 309 black truffles
N3 Reference T3 Taxonomy:39416
T4 Species 434 441 truffle
N4 Reference T4 Taxonomy:39416
T5 Species 567 574 truffle
N5 Reference T5 Taxonomy:39416
T6 Species 1416 1431 T. melanosporum
N6 Reference T6 Taxonomy:39416
T7 Species 1471 1478 truffle
N7 Reference T7 Taxonomy:39416
#1 AnnotatorNotes T4 TODO check