20970456

not annotated - annotated - LINNAEUS only

Proteotyping to establish the lineage of type A H1N1 and type B human influenza virus.

The ability to establish the lineage of type A H1N1 and type B human influenza virus strains using a new proteotyping approach is demonstrated. Lineage-specific signature peptides have been determined for the hemagglutinin antigen of type A H1N1 and type B influenza viruses. The detection of these peptides alone within the high resolution mass spectra of whole antigen digests enables the lineage of the strain to be rapidly and unequivocally assigned. This proteotyping approach complements conventional PCR approaches and should aid in the monitoring of the evolution of the influenza virus in both humans and animals.



Ann file

T1	Species 41 52;70 85	type A H1N1 influenza virus

N1 Reference T1 Taxonomy:11320

T2 Species 57 85 type B human influenza virus

N2 Reference T2 Taxonomy:11520

T3 Species 129 140;158 173 type A H1N1 influenza virus

N3 Reference T3 Taxonomy:11320

T4 Species 145 173 type B human influenza virus

N4 Reference T4 Taxonomy:11520

T5 Species 323 334;346 363 type A H1N1 influenza viruses

N5 Reference T5 Taxonomy:11320

T6 Species 339 363 type B influenza viruses

N6 Reference T6 Taxonomy:11520

T7 Out-of-scope 668 683 influenza virus

T8 Species 692 698 humans

N8 Reference T8 Taxonomy:9606

N9 Reference T7 Taxonomy:11308 Orthomyxoviridae