20970457
not annotated - annotated - LINNAEUS only
Development of an IgM-capture ELISA for Coxsackievirus A16 infection.
Diagnosis of Coxsackievirus A16 (CA16) infection in China relies mainly on reverse transcription-polymerase chain reaction (RT-PCR) that require expensive equipment and special trained personnel, thus making its wide application in health care settings unlikely. In this study, a novel IgM anti-CA16 assay was developed for the detection of IgM antibodies to CA16 in serum. The responses and diagnostic value of IgM for the CA16 infection were assessed by testing 1970 serum samples. The results showed that sensitivity of IgM test was 84.6% (259/306, 95% CI: 80.1-88.5), and specificity in control subjects and patients with CA16 HFMD was 99.2% (1508/1520, 95% CI: 98.6-99.6) and 90.3% (14/144, 95% CI: 84.2-94.6), respectively. The IgM positive rate reached 56.3% in the sera collected within the first day after onset, increased continuously to 95.3% at day 5 to day 7 after onset, and then reached 100% after more than 8 days. The cross-reaction rate in patients infected with other non-CA16 enteroviruses was 9.7% (14/144). These results suggest that the IgM anti-CA16 assay offers a rapid, convenient, and reliable method to detect acute CA16 infections.
Ann file
T1 Species 40 58 Coxsackievirus A16
N1 Reference T1 Taxonomy:138948
T2 Species 84 102 Coxsackievirus A16
N2 Reference T2 Taxonomy:138948
T3 Species 104 108 CA16
N3 Reference T3 Taxonomy:138948
T4 Out-of-scope 366 370 CA16
N4 Reference T4 Taxonomy:138948
T5 Species 430 434 CA16
N5 Reference T5 Taxonomy:138948
T6 Species 495 499 CA16
N6 Reference T6 Taxonomy:138948
T7 Species 697 701 CA16
N7 Reference T7 Taxonomy:138948
T8 Species 1062 1066 CA16
N8 Reference T8 Taxonomy:138948
T9 Out-of-scope 1140 1144 CA16
N9 Reference T9 Taxonomy:138948
T10 Species 1215 1219 CA16
N10 Reference T10 Taxonomy:138948