20980510
not annotated - annotated - LINNAEUS only
Assembly and immunological properties of Newcastle disease virus-like particles containing the respiratory syncytial virus F and G proteins.
Human respiratory syncytial virus (RSV) is a serious respiratory pathogen in infants and young children as well as elderly and immunocompromised populations. However, no RSV vaccines are available. We have explored the potential of virus-like particles (VLPs) as an RSV vaccine candidate. VLPs composed entirely of RSV proteins were produced at levels inadequate for their preparation as immunogens. However, VLPs composed of the Newcastle disease virus (NDV) nucleocapsid and membrane proteins and chimera proteins containing the ectodomains of RSV F and G proteins fused to the transmembrane and cytoplasmic domains of NDV F and HN proteins, respectively, were quantitatively prepared from avian cells. Immunization of mice with these VLPs, without adjuvant, stimulated robust, anti-RSV F and G protein antibody responses. IgG2a/IgG1 ratios were very high, suggesting predominantly T(H)1 responses. In contrast to infectious RSV immunization, neutralization antibody titers were robust and stable for 4 months. Immunization with a single dose of VLPs resulted in the complete protection of mice from RSV replication in lungs. Upon RSV intranasal challenge of VLP-immunized mice, no enhanced lung pathology was observed, in contrast to the pathology observed in mice immunized with formalin-inactivated RSV. These results suggest that these VLPs are effective RSV vaccines in mice, in contrast to other nonreplicating RSV vaccine candidates.
Ann file
T1 Species 41 64 Newcastle disease virus
N1 Reference T1 Taxonomy:11176
T2 Species 95 122 respiratory syncytial virus
N2 Reference T2 Taxonomy:12814
T3 Species 143 176 Human respiratory syncytial virus
N3 Reference T3 Taxonomy:11250
T4 Species 178 181 RSV
N4 Reference T4 Taxonomy:11250
T5 Species 313 316 RSV
N5 Reference T5 Taxonomy:11250
T6 Species 409 412 RSV
N6 Reference T6 Taxonomy:11250
T7 Species 458 461 RSV
N7 Reference T7 Taxonomy:11250
T8 Species 573 596 Newcastle disease virus
N8 Reference T8 Taxonomy:11176
T9 Species 598 601 NDV
N9 Reference T9 Taxonomy:11176
T10 Species 689 692 RSV
N10 Reference T10 Taxonomy:11250
T11 Species 764 767 NDV
N11 Reference T11 Taxonomy:11176
T12 Species 864 868 mice
N12 Reference T12 Taxonomy:10090
T13 Out-of-scope 928 931 RSV
N13 Reference T13 Taxonomy:11250
T14 Species 1070 1073 RSV
N14 Reference T14 Taxonomy:11250
T15 Species 1235 1239 mice
N15 Reference T15 Taxonomy:10090
T16 Species 1245 1248 RSV
N16 Reference T16 Taxonomy:11250
T17 Species 1276 1279 RSV
N17 Reference T17 Taxonomy:11250
T18 Species 1318 1322 mice
N18 Reference T18 Taxonomy:10090
T19 Species 1406 1410 mice
N19 Reference T19 Taxonomy:10090
T20 Species 1447 1450 RSV
N20 Reference T20 Taxonomy:11250
T21 Species 1504 1507 RSV
N21 Reference T21 Taxonomy:11250
T22 Species 1520 1524 mice
N22 Reference T22 Taxonomy:10090
T23 Species 1562 1565 RSV
N23 Reference T23 Taxonomy:11250