20980513
not annotated - annotated - LINNAEUS only
R5 HIV env and vesicular stomatitis virus G protein cooperate to mediate fusion to naive CD4+ T Cells.
Naive CD4(4) T cells are resistant to both HIV R5 env and vesicular stomatitis virus G protein (VSV-G)-mediated fusion. However, viral particles carrying both HIV R5 env and VSV-G infect naive cells by an unexplained mechanism. We show that VSV-G-pseudotyped virus cannot fuse to unstimulated cells because the viral particles cannot be endocytosed. However, virions carrying both HIV R5 env and VSV-G can fuse because CD4 binding allows viral uptake. Our findings reveal a unique mechanism by which R5 HIV env and VSV-G cooperate to allow entry to naive CD4(+) T cells, providing a tool to target naive CD4(+) T cells with R5 HIV to study HIV coreceptor signaling and latency.
Ann file
T1 Species 3 6 HIV
N1 Reference T1 Taxonomy:12721
T2 Species 15 41 vesicular stomatitis virus
N2 Reference T2 Taxonomy:11276
T3 Species 148 151 HIV
N3 Reference T3 Taxonomy:12721
T4 Species 163 189 vesicular stomatitis virus
N4 Reference T4 Taxonomy:11276
T5 Species 201 204 VSV
N5 Reference T5 Taxonomy:11276
T6 Species 234 239 viral
N6 Reference T6 Taxonomy:12037
T7 Species 264 267 HIV
N7 Reference T7 Taxonomy:12721
T8 Species 279 282 VSV
N8 Reference T8 Taxonomy:11276
T9 Species 346 349 VSV
N9 Reference T9 Taxonomy:11276
T10 Out-of-scope 364 369 virus
N10 Reference T10 Taxonomy:12037
T11 Out-of-scope 416 421 viral
N11 Reference T11 Taxonomy:12037
T12 Species 486 489 HIV
N12 Reference T12 Taxonomy:12721
T13 Species 501 504 VSV
N13 Reference T13 Taxonomy:11276
T14 Out-of-scope 543 548 viral
N14 Reference T14 Taxonomy:12037
T15 Species 608 611 HIV
N15 Reference T15 Taxonomy:12721
T16 Species 620 623 VSV
N16 Reference T16 Taxonomy:11276
T17 Species 732 735 HIV
N17 Reference T17 Taxonomy:12721
T18 Species 745 748 HIV
N18 Reference T18 Taxonomy:12721
#1 AnnotatorNotes T10 TODO check
#2 AnnotatorNotes T11 TODO check
#3 AnnotatorNotes T14 TODO check