21029103
not annotated - annotated - LINNAEUS only
Phylloplane location of glucosinolates in Barbarea spp. (Brassicaceae) and misleading assessment of host suitability by a specialist herbivore.
Glucosinolates are plant secondary metabolites used in host plant recognition by insects specialized on Brassicaceae, such as the diamondback moth (DBM), Plutella xylostella. Their perception as oviposition cues by females would seem to require their occurrence on the leaf surface, yet previous studies have reached opposite conclusions about whether glucosinolates are actually present on the surface of crucifer leaves. DBM oviposits extensively on Barbarea vulgaris, despite its larvae not being able to survive on this plant because of its content of feeding-deterrent saponins. Glucosinolates and saponins in plant tissue and mechanically removed surface waxes from leaves of Barbarea spp. were analyzed with high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS). Surface waxes from leaves of Barbarea spp. contained glucosinolates, but not feeding-deterrent saponins. Our research is the first to show that glucosinolates are present on the leaf surface of Barbarea spp., but not in other crucifers investigated, resolving some conflicting results from previous studies. Our research is also the first to quantify glucosinolates on the leaf surface of a crucifer, and to show that the concentrations of glucosinolates found on the leaf surface of Barbarea spp. are sufficient to be perceived by ovipositing DBM.
Ann file
T1 Species 276 292 diamondback moth
N1 Reference T1 Taxonomy:51655
T2 Species 294 297 DBM
N2 Reference T2 Taxonomy:51655
T3 Species 300 319 Plutella xylostella
N3 Reference T3 Taxonomy:51655
T4 Species 569 572 DBM
N4 Reference T4 Taxonomy:51655
T5 Species 598 615 Barbarea vulgaris
N5 Reference T5 Taxonomy:50459
T6 Species 1504 1507 DBM
N6 Reference T6 Taxonomy:51655