21029751
not annotated - annotated - LINNAEUS only
Real time PCR method for simultaneous detection, quantitation and differentiation of capripoxviruses.
The genus Capripoxvirus (CaPV) comprises three members namely, sheep poxvirus (SPPV), goat poxvirus (GTPV) and lumpy skin disease virus (LSDV) affecting sheep, goats and cattle, respectively. CaPV infections produce similar symptoms in sheep and goats, and the three viruses cannot be distinguished serologically. Since there are conflicting opinions regarding the host specificity of CaPVs, particularly for goatpox and sheeppox viruses, the development of rapid genotyping tools will facilitate more accurate disease diagnosis and surveillance for better management of capripox outbreaks. This paper describes a species-specific, real time polymerase chain reaction (PCR), based on unique molecular markers that were found in the G-protein-coupled chemokine receptor (GPCR) gene sequences of CaPVs, that uses dual hybridization probes for their simultaneous detection, quantitation and genotyping. The assay can differentiate between CaPV strains based on differences in the melting point temperature (Tm) obtained after fluorescence melting curve analysis (FMCA). It is highly sensitive and presents low intra- and inter-run variation. This real time PCR assay will make a significant contribution to CaPV diagnosis and to the better understanding of the epidemiology of CaPVs by enabling rapid genotyping and gene-based classification of viral strains and unequivocal identification of isolates.
Ann file
T1 Species 167 181 sheep poxvirus
N1 Reference T1 Taxonomy:10266
T2 Species 183 187 SPPV
N2 Reference T2 Taxonomy:10266
T3 Species 190 203 goat poxvirus
N3 Reference T3 Taxonomy:186805
T4 Species 205 209 GTPV
N4 Reference T4 Taxonomy:186805
T5 Species 215 239 lumpy skin disease virus
N5 Reference T5 Taxonomy:59509
T6 Species 241 245 LSDV
N6 Reference T6 Taxonomy:59509
T7 Species 257 262 sheep
N7 Reference T7 Taxonomy:9940
T8 Species 264 269 goats
N8 Reference T8 Taxonomy:9925
T9 Species 274 280 cattle
N9 Reference T9 Taxonomy:9913
T10 Species 340 345 sheep
N10 Reference T10 Taxonomy:9940
T11 Species 350 355 goats
N11 Reference T11 Taxonomy:9925
T12 Species 513 520 goatpox
N12 Reference T12 Taxonomy:186805
T13 Species 525 541 sheeppox viruses
N13 Reference T13 Taxonomy:10266
#1 AnnotatorNotes T12 TODO check span: virus related