21029752
not annotated - annotated - LINNAEUS only
Multiplex PCR for rapid detection of serotype A foot-and-mouth disease virus variants with amino acid deletion at position 59 of the capsid protein VP3.
In India, there has been co-circulation, extinction and emergence of genotypes/lineages within serotype A foot-and-mouth disease (FMD) virus. At present an antigenically heterogeneous, unique lineage within genotype VII dominates the field outbreaks. This genetic cluster has amino acid deletion at position 59 of VP3 (VP3(59)-deletion group), considered to be critical antigenically. The emergence of this group warrants rapid and accurate detection to facilitate early planning and implementation of an effective control policy. A rapid multiplex PCR assay was developed for detection of the dominating VP3(59)-deletion group with 100% sensitivity and specificity, even before generating sequence data and confirmatory phylogenetic analysis. This development is important for surveillance of FMD in India.
Ann file
T1 Species 37 76 serotype A foot-and-mouth disease virus
N1 Reference T1 Taxonomy:12110
T2 Species 285 288 FMD
N2 Reference T2 Taxonomy:12110
T3 Species 949 952 FMD
N3 Reference T3 Taxonomy:12110
#1 AnnotatorNotes T2 TODO check span: virus related