21034772
not annotated - annotated - LINNAEUS only
Multiplex RT-PCR for rapid detection and differentiation of class I and class II Newcastle disease viruses.
A multiplex RT-PCR was developed for detection and differentiation of class I and class II strains of Newcastle disease virus (NDV). The method was shown to have high specificity and sensitivity. The results obtained from the multiplex RT-PCR for a total of 67 NDV field isolates obtained in 2009 were consistent with those obtained by nucleotide sequencing and phylogenetic analysis. A phylogenetic tree based on the partial sequences of the F gene revealed that the 67 field isolates of NDV could be divided into two classes. Twenty-seven NDV isolates were grouped into class I, and two genotypes were identified. Most of the class I isolates were determined to be of genotype 3, with the exception of isolate NDV09-034, which belonged to genotype 2. Forty class II NDV isolates were divided into three genotypes, namely genotype VII (27 isolates), genotype I (2 isolates) and genotype II (11 isolates). Isolates of genotypes I and II in class II were shown to be related to commercial vaccine strains used commonly in China. All isolates of genotype VII were predicted to be virulent, on the basis of the sequence motif at the cleavage site of the F gene. This genotype has become predominantly responsible for most outbreaks of ND in China in recent years. In conclusion, this multiplex RT-PCR provides a new assay for rapid detection and differentiation of both classes of NDV isolates.
Ann file
T1 Species 60 67 class I
N1 Reference T1 Taxonomy:11176
T2 Species 72 106 class II Newcastle disease viruses
N2 Reference T2 Taxonomy:11176
T3 Species 180 187 class I
N3 Reference T3 Taxonomy:11176
T4 Species 192 241 class II strains of Newcastle disease virus (NDV)
N4 Reference T4 Taxonomy:11176
T5 Species 371 374 NDV
N5 Reference T5 Taxonomy:11176
T6 Species 599 602 NDV
N6 Reference T6 Taxonomy:11176
T7 Species 651 654 NDV
N7 Reference T7 Taxonomy:11176
T8 Species 682 689 class I
N8 Reference T8 Taxonomy:11176
T9 Species 738 745 class I
N9 Reference T9 Taxonomy:11176
T10 Species 822 831 NDV09-034
N10 Reference T10 Taxonomy:11176
T11 Species 869 890 class II NDV isolates
N11 Reference T11 Taxonomy:11176
T12 Species 1488 1491 NDV
N12 Reference T12 Taxonomy:11176
#1 AnnotatorNotes T1 TODO check
#2 AnnotatorNotes T3 TODO check
#3 AnnotatorNotes T8 TODO check