21047955
not annotated - annotated - LINNAEUS only
Alphacoronavirus transmissible gastroenteritis virus nsp1 protein suppresses protein translation in mammalian cells and in cell-free HeLa cell extracts but not in rabbit reticulocyte lysate.
The nsp1 protein of transmissible gastroenteritis virus (TGEV), an alphacoronavirus, efficiently suppressed protein synthesis in mammalian cells. Unlike the nsp1 protein of severe acute respiratory syndrome coronavirus, a betacoronavirus, the TGEV nsp1 protein was unable to bind 40S ribosomal subunits or promote host mRNA degradation. TGEV nsp1 also suppressed protein translation in cell-free HeLa cell extract; however, it did not affect translation in rabbit reticulocyte lysate (RRL). Our data suggested that HeLa cell extracts and cultured host cells, but not RRL, contain a host factor(s) that is essential for TGEV nsp1-induced translational suppression.
Ann file
T1 Species 17 52 transmissible gastroenteritis virus
N1 Reference T1 Taxonomy:693997
T2 Species 163 169 rabbit
N2 Reference T2 Taxonomy:9986
T3 Species 213 248 transmissible gastroenteritis virus
N3 Reference T3 Taxonomy:693997
T4 Species 250 254 TGEV
N4 Reference T4 Taxonomy:693997
T5 Species 366 411 severe acute respiratory syndrome coronavirus
N5 Reference T5 Taxonomy:694009
T6 Species 436 440 TGEV
N6 Reference T6 Taxonomy:693997
T7 Species 530 534 TGEV
N7 Reference T7 Taxonomy:693997
T8 Species 650 656 rabbit
N8 Reference T8 Taxonomy:9986
T9 Species 812 816 TGEV
N9 Reference T9 Taxonomy:693997