21073977
not annotated - annotated - LINNAEUS only
Cleavage of resveratrol in fungi: characterization of the enzyme Rco1 from Ustilago maydis.
Ustilago maydis, the causative agent of corn smut disease, contains two genes encoding members of the carotenoid cleavage oxygenase family, a group of enzymes that cleave double bonds in different substrates. One of them, Cco1, was formerly identified as a Beta-carotene cleaving enzyme. Here we elucidate the function of the protein encoded by the second gene, termed here as Ustilago maydis Resveratrol cleavage oxygenase 1 (Um Rco1). In vitro incubations of heterologously expressed and purified UM Rco1 with different carotenoid and stilbene substrates demonstrate that it cleaves the interphenyl Calpha-CBeta double bond of the phytoalexin resveratrol and its derivative piceatannol. Um Rco1 exhibits a high degree of substrate specificity, as suggested by the lack of activity on carotenoids and the other resveratrol-related compounds tested. The activity of Um Rco1 was confirmed by incubation of U. maydis rco1 deletion and over-expression strains with resveratrol. Furthermore, treatment with resveratrol resulted in striking alterations of cell morphology. However, pathogenicity assays indicated that Um rco1 is largely dispensable for biotrophic development. Our work reveals Um Rco1 as the first eukaryotic resveratrol cleavage enzyme identified so far. Moreover, Um Rco1 represents a subfamily of fungal enzymes likely involved in the degradation of stilbene compounds, as suggested by the cleavage of resveratrol by homologs from Aspergillus fumigatus, Chaetomium globosum and Botryotinia fuckeliana.
Ann file
T1 Species 75 90 Ustilago maydis
N1 Reference T1 Taxonomy:5270
T2 Species 94 109 Ustilago maydis
N2 Reference T2 Taxonomy:5270
T3 Species 134 143 corn smut
N3 Reference T3 Taxonomy:5270
T4 Species 471 486 Ustilago maydis
N4 Reference T4 Taxonomy:5270
T5 Species 999 1008 U. maydis
N5 Reference T5 Taxonomy:5270
T6 Species 1540 1561 Aspergillus fumigatus
N6 Reference T6 Taxonomy:746128
T7 Species 1563 1582 Chaetomium globosum
N7 Reference T7 Taxonomy:38033
T8 Species 1587 1609 Botryotinia fuckeliana
N8 Reference T8 Taxonomy:40559
#1 AnnotatorNotes T3 TODO check