21097608

not annotated - annotated - LINNAEUS only

African 2, a clonal complex of Mycobacterium bovis epidemiologically important in East Africa.

We have identified a clonal complex of Mycobacterium bovis isolated at high frequency from cattle in Uganda, Burundi, Tanzania, and Ethiopia. We have named this related group of M. bovis strains the African 2 (Af2) clonal complex of M. bovis. Af2 strains are defined by a specific chromosomal deletion (RDAf2) and can be identified by the absence of spacers 3 to 7 in their spoligotype patterns. Deletion analysis of M. bovis isolates from Algeria, Mali, Chad, Nigeria, Cameroon, South Africa, and Mozambique did not identify any strains of the Af2 clonal complex, suggesting that this clonal complex of M. bovis is localized in East Africa. The specific spoligotype pattern of the Af2 clonal complex was rarely identified among isolates from outside Africa, and the few isolates that were found and tested were intact at the RDAf2 locus. We conclude that the Af2 clonal complex is localized to cattle in East Africa. We found that strains of the Af2 clonal complex of M. bovis have, in general, four or more copies of the insertion sequence IS6110, in contrast to the majority of M. bovis strains isolated from cattle, which are thought to carry only one or a few copies.



Ann file

T1	Species 31 50	Mycobacterium bovis

N1 Reference T1 Taxonomy:1765

T2 Species 136 155 Mycobacterium bovis

N2 Reference T2 Taxonomy:1765

T3 Species 188 194 cattle

N3 Reference T3 Taxonomy:9913

T4 Species 275 283 M. bovis

N4 Reference T4 Taxonomy:1765

T5 Species 330 338 M. bovis

N5 Reference T5 Taxonomy:1765

T6 Species 514 522 M. bovis

N6 Reference T6 Taxonomy:1765

T7 Species 701 709 M. bovis

N7 Reference T7 Taxonomy:1765

T8 Species 992 998 cattle

N8 Reference T8 Taxonomy:9913

T9 Species 1066 1074 M. bovis

N9 Reference T9 Taxonomy:1765

T10 Species 1178 1186 M. bovis

N10 Reference T10 Taxonomy:1765

T11 Species 1209 1215 cattle

N11 Reference T11 Taxonomy:9913