21126599
not annotated - annotated - LINNAEUS only
The pH regulatory factor Pac1 regulates Tri gene expression and trichothecene production in Fusarium graminearum.
Fungi manage the adaptation to extra-cellular pH through the PacC transcription factor, a key component of the pH regulatory system. PacC regulates the production of various secondary metabolites in filamentous fungi. In the important cereal pathogen Fusarium graminearum, the production of trichothecene is induced only under acidic pH conditions. Here, we examined the role of the PacC homologue from F. graminearum, FgPac1, on the regulation of trichothecene production. An FgDeltaPac1 deletion mutant was constructed in F. graminearum which showed a reduced development under neutral and alkaline pH, increased sensitivity to H(2)O(2) and an earlier Tri gene induction and toxin accumulation at acidic pH. A strain expressing the FgPac1(c) constitutively active form of Pac1 exhibited a strongly repressed Tri gene expression and reduced toxin accumulation at acidic pH. These results demonstrate that Pac1 negatively regulates Tri gene expression and toxin production in F. graminearum.
Ann file
T1 Species 92 112 Fusarium graminearum
N1 Reference T1 Taxonomy:5518
T2 Species 367 387 Fusarium graminearum
N2 Reference T2 Taxonomy:5518
T3 Species 519 533 F. graminearum
N3 Reference T3 Taxonomy:5518
T4 Species 640 654 F. graminearum
N4 Reference T4 Taxonomy:5518
T5 Species 1092 1106 F. graminearum
N5 Reference T5 Taxonomy:5518